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验证数据展示

  • WB analysis using 84655-5-RR

    WB analysis using 84655-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84655-5-RR (CXCR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84655-5-RR

    IHC staining of mouse liver using 84655-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84655-5-RR (CXCR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of rat liver using 84655-5-RR

    WB analysis of rat liver using 84655-5-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 84655-5-RR (CXCR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84655-5-RR

    Affinity and Kinetic Characterization of 84655-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84655-5-RR against Human CXCR3 were performed. The affinity constant is 1.84 nM.

CXCR3 Recombinant antibody

CXCR3 Recombinant Antibody for WB, IHC, ELISA
Cat No. 84655-5-RR

产品说明书

CloneNo. 241790E9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

CMKAR3, CKR-L2, CKR L2, CD183, CD182

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84655-5-RR targets CXCR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 3
别名 CMKAR3, CKR-L2, CKR L2, CD183, CD182
计算分子量 368 aa, 41 kDa
观测分子量45 kDa
GenBank蛋白编号BC034403
基因名称 CXCR3
Gene ID (NCBI) 2833
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP49682
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of several cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. The binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively, spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as a functional receptor for CXCL4 (PMID:12782716).

实验方案

Product Specific Protocols
WB protocol for CXCR3 antibody 84655-5-RRDownload protocol
IHC protocol for CXCR3 antibody 84655-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols