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CXCR3 Recombinant antibody

CXCR3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

241790E9

Cat no : 84655-5-RR

Print datasheet

Synonyms

CMKAR3, CKR-L2, CKR L2, CD183, CD182

验证数据展示

  • WB analysis using 84655-5-RR

    WB analysis using 84655-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84655-5-RR (CXCR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 84655-5-RR

    IHC staining of mouse liver using 84655-5-RR

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84655-5-RR (CXCR3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of rat liver using 84655-5-RR

    WB analysis of rat liver using 84655-5-RR

    rat liver tissue were subjected to SDS PAGE followed by western blot with 84655-5-RR (CXCR3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Affinity and Kinetic Characterization of 84655-5-RR

    Affinity and Kinetic Characterization of 84655-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84655-5-RR against Human CXCR3 were performed. The affinity constant is 1.84 nM.

经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, K-562 cells, rat liver tissue
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84655-5-RR targets CXCR3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) receptor 3
Synonyms CMKAR3, CKR-L2, CKR L2, CD183, CD182
Calculated Molecular Weight 368 aa, 41 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC034403
Gene Symbol CXCR3
Gene ID (NCBI) 2833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP49682
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of several cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. The binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively, spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as a functional receptor for CXCL4 (PMID:12782716).

实验方案

Product Specific Protocols
WB protocol for CXCR3 antibody 84655-5-RRDownload protocol
IHC protocol for CXCR3 antibody 84655-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols