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  • Featured Product
  • KD/KO Validated

CXCR3B-specific Monoclonal antibody, PBS Only

CXCR3B-specific Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1B2D6

Cat no : 60065-1-PBS

Print datasheet

Synonyms

CXCR3, CMKAR3, CKR-L2, CKR L2, CD183

验证数据展示

  • IF Staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    IF Staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CXCR3B-specific antibody (60065-1-Ig, Clone: 1B2D6 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM004). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

    WB analysis of MOLT-4 using 60065-1-Ig (same clone as 60065-1-PBS)

    MOLT-4 cells were subjected to SDS PAGE followed by western blot with 60065-1-Ig (CXCR3B-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    IHC staining of human tonsillitis using 60065-1-Ig (same clone as 60065-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60065-1-Ig (CXCR3B-specific antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60065-1-PBS in a different storage buffer formulation.

产品信息

60065-1-PBS targets CXCR3B-specific in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name chemokine (C-X-C motif) receptor 3
Synonyms CXCR3, CMKAR3, CKR-L2, CKR L2, CD183
Calculated Molecular Weight 46 kDa
Observed Molecular Weight43 kDa
GenBank Accession NumberAF469635
Gene Symbol CXCR3
Gene ID (NCBI) 2833
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49682
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CXCR3 (C-X-C chemokine receptor type 3) is a G protein-coupled, seven-transmembrane domain chemokine receptor that is expressed on the surface of a number of cell types, including activated CD4+ and CD8+ T cells, NK and NK-T cells, plasmacytoid dendritic cells, and some B cells (PMID: 16847335). CXCR3 binds and is activated by the three IFN-gamma-inducible chemokines: CXCL9, CXCL10, and CXCL11. Binding of these ligands to CXCR3 has been implicated in regulating T-cell infiltration during inflammation and tissue injury (PMID: 17250586). Alternatively spliced transcript variants encoding different isoforms have been found for this gene. One of the isoforms, CXCR3B, mediates the angiostatic activity of CXCR3 ligands and also acts as functional receptor for CXCL4 (PMID:12782716). This monoclonal antibody is specific to CXCR3B.