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验证数据展示

  • IF Staining of mouse testis using 84904-1-RR

    IF Staining of mouse testis using 84904-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Jurkat using 84904-1-RR

    IF Staining of Jurkat using 84904-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • IF Staining of Jurkat using 84904-1-RR

    IF Staining of Jurkat using 84904-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR4 antibody (84904-1-RR, Clone: 241734B2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CXCR4 Recombinant antibody

CXCR4 Uni-rAbTM Recombinant Antibody for IF/ICC, IF-P, ELISA
Cat No. 84904-1-RR

产品说明书

CloneNo. 241734B2

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IF/ICC, IF-P, ELISA

别名

241734B2, CD184, C-X-C chemokine receptor type 4, CXC R4, CXCR 4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive IF-P detected inmouse testis tissue
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy CXCR4 Ready-To-Use IHC Kit. CXCR4 primary antibody included.

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)-PIF-P : 1:125-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84904-1-RR targets CXCR4 in IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IF/ICC, IF-P, ELISA Application Description
经测试反应性 human, mouse
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 4
别名 241734B2, CD184, C-X-C chemokine receptor type 4, CXC R4, CXCR 4
计算分子量 352 aa, 40 kDa
GenBank蛋白编号BC020968
基因名称 CXCR4
Gene ID (NCBI) 7852
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP61073
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

C-X-C chemokine receptor type 4 (CXCR4) is a widely expressed G protein-coupled seven-transmembrane receptor. CXCL12/SDF-1 is the biological ligand for CXCR4. The binding of CXCL12 to CXCR4 induces intracellular signaling through several divergent pathways initiating signals related to chemotaxis, cell survival and/or proliferation, increase in intracellular calcium, and gene transcription (PMID: 20484021). CXCR4 also functions as a coreceptor for HIV-1 entry (PMID: 9427609). CXCR4 has a calculated molecular weight of 40 kDa. In addition to the predicted species of 45-47 kDa for glycosylated CXCR4 monomers, due to ubiquitination, dimeration, and different degrees of glycosylation, additional species can also exist and have been reported in some research, including 67 kDa (PMID: 23917520), 55, 67, 87 kDa (PMID: 20028517), 80 kDa (PMID: 10506573), 47, 50, 62, and 98 kDa (PMID: 16204649).

实验方案

Product Specific Protocols
IF protocol for CXCR4 antibody 84904-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols