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CXCR5 Polyclonal antibody

CXCR5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 28239-1-AP

Print datasheet

Synonyms

MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1

验证数据展示

  • WB analysis using 28239-1-AP

    WB analysis using 28239-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28239-1-AP (CXCR5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 28239-1-AP

    IHC staining of mouse brain using 28239-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28239-1-AP (CXCR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of Jurkat using 28239-1-AP

    WB analysis of Jurkat using 28239-1-AP

    WB result of CXCR5 antibody (28239-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CXCR5 transfected Jurkat cells.

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, mouse spleen tissue, rat spleen tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28239-1-AP targets CXCR5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CXCR5 fusion protein Ag28271 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chemokine (C-X-C motif) receptor 5
Synonyms MDR 15, CXCR 5, CXC R5, CD185, Burkitt lymphoma receptor 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight33 kDa
GenBank Accession NumberNM_001716
Gene Symbol CXCR5
Gene ID (NCBI) 643
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP32302
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Initially discovered in 1992 as Burkitt's lymphoma receptor 1, CXCR5 (chemokine receptor 5, also known as CD185) is a seven-transmembrane G protein-coupled receptor (GPCR) protein (PMID: 32994482). CXCR5 is essential for naïve B-cell migration to and maturation in the lymph nodes and spleen, as well as for cluster of differentiation 4 T-cell (TFH and TCM) homing and interaction with B cells (PMID: 21471443). Moreover, CXCR5 is involved in the regulation of neural stem cells (NSC) and neuronal regeneration in the mouse brain, neuronal differentiation, neurogenesis, gliogenesis, and synaptogenesis (PMID: 37460877).

实验方案

Product Specific Protocols
WB protocol for CXCR5 antibody 28239-1-APDownload protocol
IHC protocol for CXCR5 antibody 28239-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols