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CXCR7 Recombinant antibody

CXCR7 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

240842C9

Cat no : 83927-1-RR

Print datasheet

Synonyms

ACKR3, 240842C9, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1

验证数据展示

  • WB analysis using 83927-1-RR

    WB analysis using 83927-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83927-1-RR (CXCR7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 83927-1-RR

    IF Staining of Jurkat using 83927-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR7 antibody (83927-1-RR, Clone: 240842C9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Raji cells, U-87 MG cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 83927-1-RR is CXCR7 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen CXCR7 fusion protein Ag14247 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name chemokine (C-X-C motif) receptor 7
Synonyms ACKR3, 240842C9, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1
Calculated Molecular Weight 362 aa, 41 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC036661
Gene Symbol CXCR7
Gene ID (NCBI) 57007
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP25106
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a G-protein coupled receptor family member. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. The expression of CXCR7 has been associated with cardiac development, tumor growth, and progression.

实验方案

Product Specific Protocols
WB protocol for CXCR7 antibody 83927-1-RRDownload protocol
IF protocol for CXCR7 antibody 83927-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols