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验证数据展示

  • WB analysis using 83927-1-RR

    WB analysis using 83927-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83927-1-RR (CXCR7 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 83927-1-RR

    IF Staining of Jurkat using 83927-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using CXCR7 antibody (83927-1-RR, Clone: 240842C9 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

CXCR7 Recombinant antibody

CXCR7 Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 83927-1-RR

产品说明书

CloneNo. 240842C9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

ACKR3, 240842C9, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells, K-562 cells, Raji cells, U-87 MG cells
Positive IF/ICC detected inJurkat cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83927-1-RR targets CXCR7 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 CXCR7 fusion protein Ag14247 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 chemokine (C-X-C motif) receptor 7
别名 ACKR3, 240842C9, C-X-C chemokine receptor type 7, CMKOR1, Chemokine orphan receptor 1
计算分子量 362 aa, 41 kDa
观测分子量50 kDa
GenBank蛋白编号BC036661
基因名称 CXCR7
Gene ID (NCBI) 57007
RRIDAB_3671507
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP25106
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXCR7 (C-X-C chemokine receptor type 7), also known as RDC1, is a G-protein coupled receptor family member. CXCR7 can bind the chemokines CXCL11 and CXCL12 with high affinity, and it also acts as a coreceptor with CXCR4 for a restricted number of HIV isolates. The expression of CXCR7 has been associated with cardiac development, tumor growth, and progression.

实验方案

Product Specific Protocols
WB protocol for CXCR7 antibody 83927-1-RRDownload protocol
IF protocol for CXCR7 antibody 83927-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols