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  • Featured Product
  • KD/KO Validated

CXXC5 Polyclonal antibody

CXXC5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 16513-1-AP

Print datasheet

Synonyms

Putative NF-kappa-B-activating protein 102, Putative MAPK-activating protein PM08, HSPC195, CXXC-type zinc finger protein 5, CXXC finger 5

验证数据展示

  • WB analysis of mouse brain using 16513-1-AP

    WB analysis of mouse brain using 16513-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 16513-1-AP

    WB analysis using 16513-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 16513-1-AP (CXXC5 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

  • IP experiment of SH-SY5Y using 16513-1-AP

    IP experiment of SH-SY5Y using 16513-1-AP

    IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with SH-SY5Y cells lysate 1280 ug.

  • IP experiment of SH-SY5Y using 16513-1-AP

    IP experiment of SH-SY5Y using 16513-1-AP

    IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:300) with SH-SY5Y cells lysate 3400 ug.

  • IHC staining of human prostate cancer using 16513-1-AP

    IHC staining of human prostate cancer using 16513-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IP experiment of mouse brain using 16513-1-AP

    IP experiment of mouse brain using 16513-1-AP

    IP result of anti-CXXC5 (IP:16513-1-AP, 4ug; Detection:16513-1-AP 1:6000) with mouse brain tissue lysate 2400 ug.

  • IHC staining of mouse brain using 16513-1-AP

    IHC staining of mouse brain using 16513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IHC staining of mouse brain using 16513-1-AP

    IHC staining of mouse brain using 16513-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IHC staining of rat brain using 16513-1-AP

    IHC staining of rat brain using 16513-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 16513-1-AP (CXXC5 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CXXC5 antibody included
  • IF Staining of MCF-7 using 16513-1-AP

    IF Staining of MCF-7 using 16513-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CXXC5 antibody (16513-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

经过测试的应用

Positive WB detected inMCF-7 cells, mouse brain tissue, SH-SY5Y cells
Positive IP detected inSH-SY5Y cells, mouse brain tissue
Positive IHC detected inmouse brain tissue, human prostate cancer tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy CXXC5 Ready-To-Use IHC Kit. CXXC5 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16513-1-AP targets CXXC5 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, ChIP
Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, zebrafish
Immunogen CXXC5 fusion protein Ag9733 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name CXXC finger 5
Synonyms Putative NF-kappa-B-activating protein 102, Putative MAPK-activating protein PM08, HSPC195, CXXC-type zinc finger protein 5, CXXC finger 5
Calculated Molecular Weight322aa,33 kDa; 227aa,24 kDa
Observed Molecular Weight 35-40 kDa
GenBank Accession NumberBC017439
Gene Symbol CXXC5
Gene ID (NCBI) 51523
RRIDAB_2878269
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7LFL8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CXXC5 (CXXC-type zinc finger protein 5) is also named as HSPC195, RINF and TCCCIA00297.CXXC5 is a member of the CXXC-type zinc-finger protein family. CXXC5 is a short protein (322 amino acids in length) that does not have any catalytic domain, but is able to bind to DNA and act as a transcription factor and epigenetic factor through protein-protein interactions. In addition, CXXC5 is capable of regulating various signal transduction processes, including the TGF-β, Wnt and ATM-p53 pathways, thereby acting as a novel and crucial signaling coordinator. CXXC5 plays an important role in embryonic development and adult tissue homeostasis by regulating cell proliferation, differentiation and apoptosis (PMID: 30479059). CXXC5 has two isoforms of 33 kDa and 24 kDa (PMID: 25805812). CXXC5 is modified by phosphorylation after translation.

实验方案

Product Specific Protocols
WB protocol for CXXC5 antibody 16513-1-APDownload protocol
IHC protocol for CXXC5 antibody 16513-1-APDownload protocol
IF protocol for CXXC5 antibody 16513-1-APDownload protocol
IP protocol for CXXC5 antibody 16513-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanChIP,IHC,WB

Nat Commun

A noncanonical AR addiction drives enzalutamide resistance in prostate cancer.

Authors - Yundong He
  • KD Validated
View Article
mouseWB

J Exp Med

Epigenetic regulator CXXC5 recruits DNA demethylase Tet2 to regulate TLR7/9-elicited IFN response in pDCs.

Authors - Shixin Ma
  • KO Validated
View Article
humanWB

Oncogene

NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.

Authors - Sheng Tan
  • KD Validated
View Article
humanWB,ChIP

Haematologica

The epigenetic regulator RINF (CXXC5) maintains SMAD7 expression in human immature erythroid cells and sustains red blood cells expansion.

Authors - Audrey Astori
View Article
humanWB

Sci Rep

KANK1 inhibits cell growth by inducing apoptosis though regulating CXXC5 in human malignant peripheral nerve sheath tumors.

Authors - Zhibin Cui
View Article
human,mouseWB,ChIP

Front Cell Dev Biol

Down-Regulation of CXXC5 De-Represses MYCL1 to Promote Hepatic Stellate Cell Activation.

Authors - Xiaoyan Wu
View Article