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验证数据展示

  • IHC staining of human breast cancer using 26735-1-AP

    IHC staining of human breast cancer using 26735-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26735-1-AP (CYBRD1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 26735-1-AP

    IHC staining of human breast cancer using 26735-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 26735-1-AP (CYBRD1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis of COLO 320 using 26735-1-AP

    WB analysis of COLO 320 using 26735-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 26735-1-AP (CYBRD1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

CYBRD1 Polyclonal antibody

CYBRD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 26735-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, IHC, ELISA

别名

CYBRD1, cytochrome b reductase 1, DCYTB, Duodenal cytochrome b, Ferric chelate reductase 3, FRRS3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26735-1-AP targets CYBRD1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, mouse, pig
免疫原 CYBRD1 fusion protein Ag24968 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome b reductase 1
别名 CYBRD1, cytochrome b reductase 1, DCYTB, Duodenal cytochrome b, Ferric chelate reductase 3, FRRS3
观测分子量 25 kDa
GenBank蛋白编号BC065290
基因名称 CYBRD1
Gene ID (NCBI) 79901
RRIDAB_2880618
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ53TN4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYBRD1 antibody 26735-1-APDownload protocol
IHC protocol for CYBRD1 antibody 26735-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
pigWB

Metallomics

Tolerable upper intake level of iron damages the intestine and alters the intestinal flora in weaned piglets.

Authors - Haoxuan Ding
View Article
mouseWB

Biol Trace Elem Res

Chronic Psychological Stress Disrupts Iron Metabolism and Enhances Hepatic Mitochondrial Function in Mice.

Authors - Shihui Guo
View Article
humanWB, IHC

PeerJ

Identification of potential genes in upper tract urothelial carcinoma using next-generation sequencing with bioinformatics and in vitro analyses.

Authors - Hsiang-Ying Lee
View Article