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验证数据展示

  • WB analysis using 27925-1-AP

    WB analysis using 27925-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27925-1-AP (CYFIP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27925-1-AP

    IF Staining of HeLa using 27925-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYFIP1 antibody (27925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

CYFIP1 Polyclonal antibody

CYFIP1 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 27925-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

CYFIP1, KIAA0068, P140SRA 1, SHYC, Sra 1, SRA1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27925-1-AP targets CYFIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 Human, mouse, rat
免疫原 CYFIP1 fusion protein Ag27419 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytoplasmic FMR1 interacting protein 1
别名 CYFIP1, KIAA0068, P140SRA 1, SHYC, Sra 1, SRA1
计算分子量 1253 aa, 145 kDa
观测分子量140-145 kDa
GenBank蛋白编号BC005097
基因名称 CYFIP1
Gene ID (NCBI) 23191
RRIDAB_3086011
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ7L576
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYFIP1 is a protein that regulates cytoskeletal dynamics and protein translation. The encoded protein is a component of the WAVE regulatory complex (WRC), which promotes actin polymerization. This protein also interacts with the synaptic functional regulator FMR1 protein and translation initiation factor 4E to inhibit protein translation. A large chromosomal deletion including this gene is associated with increased risk of schizophrenia and epilepsy in human patients. Reduced expression of this gene has been observed in various human cancers and the encoded protein may inhibit tumor invasion.

实验方案

Product Specific Protocols
WB protocol for CYFIP1 antibody 27925-1-APDownload protocol
IF protocol for CYFIP1 antibody 27925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols