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CYFIP1 Polyclonal antibody

CYFIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 27925-1-AP

Print datasheet

Synonyms

CYFIP1, KIAA0068, P140SRA 1, SHYC, Sra 1, SRA1

验证数据展示

  • WB analysis using 27925-1-AP

    WB analysis using 27925-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27925-1-AP (CYFIP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 27925-1-AP

    IF Staining of HeLa using 27925-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CYFIP1 antibody (27925-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27925-1-AP targets CYFIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen CYFIP1 fusion protein Ag27419 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytoplasmic FMR1 interacting protein 1
Synonyms CYFIP1, KIAA0068, P140SRA 1, SHYC, Sra 1, SRA1
Calculated Molecular Weight 1253 aa, 145 kDa
Observed Molecular Weight140-145 kDa
GenBank Accession NumberBC005097
Gene Symbol CYFIP1
Gene ID (NCBI) 23191
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7L576
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYFIP1 is a protein that regulates cytoskeletal dynamics and protein translation. The encoded protein is a component of the WAVE regulatory complex (WRC), which promotes actin polymerization. This protein also interacts with the synaptic functional regulator FMR1 protein and translation initiation factor 4E to inhibit protein translation. A large chromosomal deletion including this gene is associated with increased risk of schizophrenia and epilepsy in human patients. Reduced expression of this gene has been observed in various human cancers and the encoded protein may inhibit tumor invasion.

实验方案

Product Specific Protocols
WB protocol for CYFIP1 antibody 27925-1-APDownload protocol
IF protocol for CYFIP1 antibody 27925-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols