• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CYLC2 Polyclonal antibody

CYLC2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18729-1-AP

Print datasheet

Synonyms

CYL2, CYLC2, Cylicin 2, Cylicin II, Multiple band polypeptide II

验证数据展示

  • WB analysis of human adrenal gland using 18729-1-AP

    WB analysis of human adrenal gland using 18729-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 18729-1-AP (CYLC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18729-1-AP

    IHC staining of human testis using 18729-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18729-1-AP (CYLC2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 18729-1-AP

    IHC staining of human testis using 18729-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18729-1-AP (CYLC2 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inhuman adrenal gland tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18729-1-AP targets CYLC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cylicin, basic protein of sperm head cytoskeleton 2
Synonyms CYL2, CYLC2, Cylicin 2, Cylicin II, Multiple band polypeptide II
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC114547
Gene Symbol CYLC2
Gene ID (NCBI) 1539
RRIDAB_2276962
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ14093
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYLC2, also named as CYL2, possible plays an architectural role during spermatogenesis. It may be involved in spermatid differentiation.

实验方案

Product Specific Protocols
WB protocol for CYLC2 antibody 18729-1-APDownload protocol
IHC protocol for CYLC2 antibody 18729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols