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验证数据展示

  • WB analysis of human adrenal gland using 18729-1-AP

    WB analysis of human adrenal gland using 18729-1-AP

    human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 18729-1-AP (CYLC2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human testis using 18729-1-AP

    IHC staining of human testis using 18729-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18729-1-AP (CYLC2 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human testis using 18729-1-AP

    IHC staining of human testis using 18729-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 18729-1-AP (CYLC2 antibody) at dilution of 1:100 (under 40x lens).

CYLC2 Polyclonal antibody

CYLC2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18729-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Multiple-band polypeptide II, CylicinII, Cylicin-2, Cylicin2, Cylicin 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman adrenal gland tissue
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18729-1-AP targets CYLC2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cylicin, basic protein of sperm head cytoskeleton 2
别名 Multiple-band polypeptide II, CylicinII, Cylicin-2, Cylicin2, Cylicin 2
计算分子量 39 kDa
观测分子量 39 kDa
GenBank蛋白编号BC114547
基因名称 CYLC2
Gene ID (NCBI) 1539
RRIDAB_2276962
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ14093
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYLC2, also named as CYL2, possible plays an architectural role during spermatogenesis. It may be involved in spermatid differentiation.

实验方案

Product Specific Protocols
WB protocol for CYLC2 antibody 18729-1-APDownload protocol
IHC protocol for CYLC2 antibody 18729-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols