• Featured Product
  • KD/KO验证

CYP19A1 Polyclonal antibody

CYP19A1 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 16554-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (2)

应用

WB, IHC, IF/ICC, IF-P, ELISA and More (1)

CYP19, CYAR, CPV1, Aromatase, ARO1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA2780 cells, human placenta tissue
Positive IHC detected inhuman placenta tissue, rat brain tissue, human kidney tissue, human skeletal muscle tissue, human gliomas tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman placenta tissue
Positive IF/ICC detected inHepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16554-1-AP targets CYP19A1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, goat, geese
免疫原 CYP19A1 fusion protein Ag9765 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 19, subfamily A, polypeptide 1
别名 CYP19, CYAR, CPV1, Aromatase, ARO1
计算分子量 20 kDa, 25 kDa, 58 kDa
观测分子量 49-55 kDa
GenBank蛋白编号BC022896
基因名称 CYP19A1
Gene ID (NCBI) 1588
RRIDAB_2088670
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP11511
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).

实验方案

Product Specific Protocols
WB protocol for CYP19A1 antibody 16554-1-APDownload protocol
IHC protocol for CYP19A1 antibody 16554-1-APDownload protocol
IF protocol for CYP19A1 antibody 16554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Clin Transl Med

Treg deficiency-mediated TH 1 response causes human premature ovarian insufficiency through apoptosis and steroidogenesis dysfunction of granulosa cells.

Authors - Xue Jiao
mouseWB

Sci Rep

Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure.

Authors - Huidan Wang
mouse, ratWB

Sci Rep

Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.

Authors - Jiansha Li
  • KD Validated
humanWB,IHC

Fertil Steril

Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome.

Authors - Miao Sun
ratWB

Environ Pollut

Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation.

Authors - Zhiliang Li
mouseWB

Endocrinology

The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin.

Authors - Qing He
Loading...