验证数据展示
经过测试的应用
Positive WB detected in | A2780 cells, human placenta tissue |
Positive IHC detected in | human placenta tissue, rat brain tissue, human kidney tissue, human skeletal muscle tissue, human gliomas tissue, human testis tissue, human spleen tissue, human lung tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | human placenta tissue |
Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16554-1-AP targets CYP19A1 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, IF/ICC, IF-P, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, goat, geese |
免疫原 | CYP19A1 fusion protein Ag9765 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cytochrome P450, family 19, subfamily A, polypeptide 1 |
别名 | CYP19, CYAR, CPV1, Aromatase, ARO1 |
计算分子量 | 20 kDa, 25 kDa, 58 kDa |
观测分子量 | 49-55 kDa |
GenBank蛋白编号 | BC022896 |
基因名称 | CYP19A1 |
Gene ID (NCBI) | 1588 |
RRID | AB_2088670 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P11511 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).
实验方案
Product Specific Protocols | |
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WB protocol for CYP19A1 antibody 16554-1-AP | Download protocol |
IHC protocol for CYP19A1 antibody 16554-1-AP | Download protocol |
IF protocol for CYP19A1 antibody 16554-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Clin Transl Med Treg deficiency-mediated TH 1 response causes human premature ovarian insufficiency through apoptosis and steroidogenesis dysfunction of granulosa cells. | ||
Sci Rep Novel WT1 Missense Mutations in Han Chinese Women with Premature Ovarian Failure. | ||
Sci Rep Estrogen Secreted by Mesenchymal Stem Cells Necessarily Determines Their Feasibility of Therapeutical Application.
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Fertil Steril Elevated maternal androgen is associated with dysfunctional placenta and lipid disorder in newborns of mothers with polycystic ovary syndrome. | ||
Environ Pollut Hormonal changes and folliculogenesis in female offspring of rats exposed to cadmium during gestation and lactation. | ||
Endocrinology The Immp2l Mutation Causes Ovarian Aging Through ROS-Wnt/β-Catenin-Estrogen Pathway: Preventive Effect of Melatonin. |