验证数据展示
经过测试的应用
Positive WB detected in | HL-60 cells, K-562 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
28379-1-AP targets CYP19A1 in WB, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | human |
免疫原 | CYP19A1 fusion protein Ag29004 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | cytochrome P450, family 19, subfamily A, polypeptide 1 |
别名 | |
观测分子量 | 58-60 kDa |
GenBank蛋白编号 | BC035959 |
基因名称 | CYP19A1 |
Gene ID (NCBI) | 1588 |
RRID | AB_3669657 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P11511 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP19A1(Cytochrome P450 19A1) is also named as ARO1(aromatase), CYAR, CYP19, estrogen synthase and belongs to the cytochrome P450 family. It is a terminal enzyme which transforms irreversibly androgens into estrogens and it is present in the endoplasmic reticulum of numerous tissues(PMID:16406261). The gene encodes a protein with the molecular weight between 46 kDa and 69 kDa(PMID:8129748) and the protein can be glycosylated, but the level of glycosylation does not seem to be essential for CYP19A1 activity(PMID:12606587). Brain aromatase may be neuroprotective by increasing the local estrogen levels in injured neurons so that genetic variation in the brain aromatase gene may modify the risk for AD(PMID:16767510).
实验方案
Product Specific Protocols | |
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WB protocol for CYP19A1 antibody 28379-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |