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CYP20A1 Monoclonal antibody, PBS Only

CYP20A1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2A9E2

Cat No : 67433-1-PBS

Print datasheet

Synonyms

CYP M, CYP20A1, Cytochrome P450 20A1

验证数据展示

  • WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

    IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

    IF Staining of HeLa using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CYP20A1 antibody (67433-1-Ig, Clone: 2A9E2 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

    IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

    IHC staining of human liver cancer using 67433-1-Ig (same clone as 67433-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67433-1-Ig (CYP20A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

  • WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    WB analysis using 67433-1-Ig (same clone as 67433-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67433-1-Ig (CYP20A1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67433-1-PBS in a different storage buffer formulation.

产品信息

67433-1-PBS targets CYP20A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CYP20A1 fusion protein Ag9715 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 20, subfamily A, polypeptide 1
Synonyms CYP M, CYP20A1, Cytochrome P450 20A1
Calculated Molecular Weight 462 aa, 52 kDa
Observed Molecular Weight 49-52 kDa
GenBank Accession NumberBC020616
Gene Symbol CYP20A1
Gene ID (NCBI) 57404
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ6UW02
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C