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CYP21A2 Monoclonal antibody

CYP21A2 Monoclonal Antibody for WB, IF, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1G2G6

Cat No : 67421-1-Ig

Print datasheet

Synonyms

CA21H, CAH1, CYP21, CYP21A2, CYP21B, P450c21B, Steroid 21-hydroxylase

验证数据展示

  • WB analysis using 67421-1-Ig

    WB analysis using 67421-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of PC-12 using 67421-1-Ig

    WB analysis of PC-12 using 67421-1-Ig

    PC-12 cells were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of pig adrenal gland using 67421-1-Ig

    WB analysis of pig adrenal gland using 67421-1-Ig

    pig adrenal gland tissue were subjected to SDS PAGE followed by western blot with 67421-1-Ig (CYP21A2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of PC-12 using 67421-1-Ig

    IF Staining of PC-12 using 67421-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed PC-12 cells using CYP21A2 antibody (67421-1-Ig, Clone: 1G2G6 ) at dilution of 1:800 and CoraLite®594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells, PC-12 cells, pig adrenal gland tissue
Positive IF detected inPC-12 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)IF : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67421-1-Ig targets CYP21A2 in WB, IF, ELISA applications and shows reactivity with Human, Pig samples.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human, Pig
Immunogen CYP21A2 fusion protein Ag26292 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 21, subfamily A, polypeptide 2
Synonyms CA21H, CAH1, CYP21, CYP21A2, CYP21B, P450c21B, Steroid 21-hydroxylase
Calculated Molecular Weight 56 kDa
Observed Molecular Weight 53-56 kDa
GenBank Accession NumberNM_000500
Gene Symbol CYP21A2
Gene ID (NCBI) 1589
RRIDAB_2882661
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP08686
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP21A2 antibody 67421-1-IgDownload protocol
IF protocol for CYP21A2 antibody 67421-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols