验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells, PC-12 cells, pig adrenal gland tissue |
| Positive IF/ICC detected in | PC-12 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67421-1-Ig targets CYP21A2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Pig samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, Pig |
| 免疫原 | CYP21A2 fusion protein Ag26292 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 21, subfamily A, polypeptide 2 |
| 别名 | CA21H, CAH1, CYP21, CYP21A2, CYP21B, P450c21B, Steroid 21-hydroxylase |
| 计算分子量 | 56 kDa |
| 观测分子量 | 53-56 kDa |
| GenBank蛋白编号 | NM_000500 |
| 基因名称 | CYP21A2 |
| Gene ID (NCBI) | 1589 |
| RRID | AB_2882661 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P08686 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP21A2 antibody 67421-1-Ig | Download protocol |
| IF protocol for CYP21A2 antibody 67421-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |