• Featured Product
  • KD/KO Validated

CYP26B1 Polyclonal antibody

CYP26B1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 21555-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA and More (1)

Cytochrome P450RAI-2, Cytochrome P450RAI 2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450 26B1, Cytochrome P450 26A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, human brain tissue, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman hepatocirrhosis tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21555-1-AP targets CYP26B1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CYP26B1 fusion protein Ag15368 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 26, subfamily B, polypeptide 1
Synonyms Cytochrome P450RAI-2, Cytochrome P450RAI 2, Cytochrome P450 retinoic acid-inactivating 2, Cytochrome P450 26B1, Cytochrome P450 26A2
Calculated Molecular Weight 512 aa, 58 kDa
Observed Molecular Weight49-58 kDa
GenBank Accession NumberBC069443
Gene Symbol CYP26B1
Gene ID (NCBI) 56603
RRIDAB_10733471
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR63
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYP26B1(Cytochrome P450 26B1) is involved in the metabolism of retinoic acid (RA), rendering this classical morphogen inactive through oxidation.It has 3 isoforms produced by alternative splicing.Cyp26b1 deficiency induces pronounced apoptosis in the developing limb and delayed chondrocyte maturation(PMID:15030763).Defects in CYP26B1 are the cause of radiohumeral fusions with other skeletal and craniofacial anomalies (RHFCA).

实验方案

Product Specific Protocols
WB protocol for CYP26B1 antibody 21555-1-APDownload protocol
IHC protocol for CYP26B1 antibody 21555-1-APDownload protocol
IF protocol for CYP26B1 antibody 21555-1-APDownload protocol
IP protocol for CYP26B1 antibody 21555-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Genet

Exome-wide analyses identify low-frequency variant in CYP26B1 and additional coding variants associated with esophageal squamous cell carcinoma.

Authors - Jiang Chang
  • KD Validated
mouseIHC

Brain Struct Funct

Activity-dependent and graded BACE1 expression in the olfactory epithelium is mediated by the retinoic acid metabolizing enzyme CYP26B1.

Authors - Hande Login
mouseIF

J Neurosci

The Stimulus-Dependent Gradient of Cyp26B1+ Olfactory Sensory Neurons Is Necessary for the Functional Integrity of the Olfactory Sensory Map.

Authors - Hande Login
mouseIF

J Neurosci

Increased Retinoic Acid Catabolism in Olfactory Sensory Neurons Activates Dormant Tissue-Specific Stem Cells and Accelerates Age-Related Metaplasia.

Authors - Sofia Håglin

Front Mol Neurosci

Transcriptomics of Environmental Enrichment Reveals a Role for Retinoic Acid Signaling in Addiction.

Authors - Yafang Zhang
humanWB

FASEB J

Dysregulated retinoic acid signaling in airway smooth muscle cells in asthma.

Authors - Amy E Defnet
Loading...