CYP27A1 Polyclonal antibody

CYP27A1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14739-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, ELISA and More (1)

Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inmouse liver tissue, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14739-1-AP targets CYP27A1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, rabbit, chicken
免疫原 CYP27A1 fusion protein Ag6408 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cytochrome P450, family 27, subfamily A, polypeptide 1
别名 Cytochrome P450 27, Cytochrome P 450C27/25, CYP27, CTX, CP27
计算分子量 60 kDa
观测分子量 60-65 kDa
GenBank蛋白编号BC051851
基因名称 CYP27A1
Gene ID (NCBI) 1593
RRIDAB_2089276
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02318
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

实验方案

Product Specific Protocols
WB protocol for CYP27A1 antibody 14739-1-APDownload protocol
IHC protocol for CYP27A1 antibody 14739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Cell Res

Tissue-specific transcription reprogramming promotes liver metastasis of colorectal cancer.

Authors - Shuaishuai Teng
IHC

J Lipid Res

Increased maternal and fetal cholesterol efflux capacity and placental CYP27A1 expression in preeclampsia.

Authors - Hiten D Mistry
ratWB

Phytomedicine

Dehydrodiconiferyl alcohol, a lignan from Herpetospermum pedunculosum, alleviates cholestasis by activating pathways associated with the farnesoid X receptor.

Authors - Xiaodong Wei
ratWB

Phytomedicine

Dolomiaea souliei ethyl acetate extract protected against α-naphthylisothiocyanate-induced acute intrahepatic cholestasis through regulation of farnesoid x receptor-mediated bile acid metabolism

Authors - FanCheng Meng
human,mouseWB,IHC

Phytomedicine

Alisol B 23-acetate adjusts bile acid metabolisim via hepatic FXR-BSEP signaling activation to alleviate atherosclerosis.

Authors - Yu Fu
WB

J Agric Food Chem

Oryzanol Attenuates High Fat and Cholesterol Diet-Induced Hyperlipidemia by Regulating the Gut Microbiome and Amino Acid Metabolism.

Authors - Sisi Yan
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