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CYP27A1 Polyclonal antibody

CYP27A1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19195-1-AP

Print datasheet

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

验证数据展示

  • WB analysis of L02 using 19195-1-AP

    WB analysis of L02 using 19195-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 19195-1-AP (CYP27A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 19195-1-AP

    IHC staining of human kidney using 19195-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19195-1-AP (CYP27A1 antibody) at dilution of 1:100 (under 10x lens).

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human kidney using 19195-1-AP

    IHC staining of human kidney using 19195-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 19195-1-AP (CYP27A1 antibody) at dilution of 1:100 (under 40x lens).

    Ready-to-use IHC kits available with CYP27A1 antibody included

经过测试的应用

Positive WB detected inL02 cells, HepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19195-1-AP targets CYP27A1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivitymouse
Immunogen CYP27A1 fusion protein Ag7746 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Synonyms CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 60 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ02318
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Mitochondrial sterol 27-hydroxylase (CYP27A1) catalyzes oxidative cleavage of the sterol side chain in the bile acid biosynthetic pathway in the liver and 27-hydroxylation of cholesterol in most tissues(PMID:17088262). It belongs to the cytochrome P450 family. CYP27A1 catalyses an important sterol elimination pathway in the macrophage, and consequently may protect against atherosclerosis.

发表文章

SpeciesApplicationTitle
mouseWB

Toxicol Sci

Hepatic, metabolic and toxicity evaluation of repeated oral administration of SnS2 nanoflowers in mice.

Authors - Disi Bai
View Article