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  • Featured Product
  • KD/KO Validated

CYP27A1 Monoclonal antibody, PBS Only

CYP27A1 Monoclonal Antibody for WB,IHC,IF,ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB,IHC,IF,ELISA

Conjugate

Unconjugated

CloneNo.

3G3F7

Cat No : 67045-1-PBS

Print datasheet

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

验证数据展示

  • WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

产品信息

67045-1-PBS targets CYP27A1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,IHC,IF,ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CYP27A1 fusion protein Ag7746 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Synonyms CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02318
Storage Buffer PBS only
Storage ConditionsStore at -80°C.