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  • Featured Product
  • KD/KO Validated

CYP27A1 Monoclonal antibody, PBS Only

CYP27A1 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

3G3F7

Cat no : 67045-1-PBS

Print datasheet

Synonyms

CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase

验证数据展示

  • WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB analysis of Caco-2 using 67045-1-Ig (same clone as 67045-1-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 67045-1-Ig (CYP27A1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB analysis of HepG2 using 67045-1-Ig (same clone as 67045-1-PBS)

    WB result of CYP27A1 antibody (67045-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP27A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IHC staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67045-1-Ig (CYP27A1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CYP27A1 antibody included
  • IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

  • IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    IF Staining of human liver cancer using 67045-1-Ig (same clone as 67045-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CYP27A1 antibody (67045-1-Ig, Clone: 3G3F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67045-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CYP27A1 Ready-To-Use IHC Kit. CYP27A1 primary antibody included.

产品信息

67045-1-PBS targets CYP27A1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen CYP27A1 fusion protein Ag7746 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cytochrome P450, family 27, subfamily A, polypeptide 1
Synonyms CP27, CTX, CYP27, CYP27A1, Cytochrome P 450C27/25, Cytochrome P450 27, Sterol 27 hydroxylase, Vitamin D(3) 25 hydroxylase
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 54-60 kDa
GenBank Accession NumberBC051851
Gene Symbol CYP27A1
Gene ID (NCBI) 1593
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ02318
Storage Buffer PBS only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C