验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human liver cancer tissue, human liver tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 6 publications below |
| IHC | See 1 publications below |
产品信息
17868-1-AP targets CYP2D6/7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CYP2D6/7 fusion protein Ag12351 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 2, subfamily D, polypeptide 6 |
| 别名 | CPD6, CYP2D, CYP2D@, CYP2D6, CYP2D6/7, CYP2DL1, Cytochrome P450 2D6, P450 DB1, P450C2D, P450DB1 |
| 计算分子量 | 497 aa, 56 kDa |
| 观测分子量 | 48-56 kDa |
| GenBank蛋白编号 | BC075023 |
| 基因名称 | CYP2D6 |
| Gene ID (NCBI) | 1565 |
| RRID | AB_2878457 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P10635 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP2D6(Cytochrome P450 2D6) is also named as CYP2DL1 and belongs to the cytochrome P450 family. It is a monooxygenase that involved in the metabolism of drugs such as antiarrhythmics, adrenoceptor antagonists, and tricyclic antidepressants. CYP2D6 is responsible for the metabolism of many drugs and environmental chemicals that it oxidizes. It has 2 isoforms produced by alternative splicing with the molecular weight of 56 kDa and 50 kDa. This antibody can recognize human CYP2D6/7, mouse CYP2D3/4/9/10/22/26, rat CYP2D1/3/4/10/26, due to the high homology.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP2D6/7 antibody 17868-1-AP | Download protocol |
| IHC protocol for CYP2D6/7 antibody 17868-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Theranostics Generation of hepatic spheroids using human hepatocyte-derived liver progenitor-like cells for hepatotoxicity screening. | ||
Drug Metab Pharmacokinet Cloning, sequence analysis, and tissue expression of marmoset paraoxonase 1. | ||
Xenobiotica Comparison of mouse and human cytochrome P450 mediated-drug metabolising activities in hepatic and extrahepatic microsomes. | ||
Pharmazie Silybin enhances the blood concentration of brexpiprazole in rats by downregulating expression of CYP3A4 and CYP2D6. | ||
