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CYP2F1 Polyclonal antibody

CYP2F1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21579-1-AP

Print datasheet

Synonyms

C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1

验证数据展示

  • WB analysis of mouse lung using 21579-1-AP

    WB analysis of mouse lung using 21579-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 21579-1-AP is CYP2F1 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP2F1 fusion protein Ag16124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 2, subfamily F, polypeptide 1
Synonyms C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC109056
Gene Symbol CYP2F1
Gene ID (NCBI) 1572
RRIDAB_10733220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24903
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP2F1 antibody 21579-1-APDownload protocol
IHC protocol for CYP2F1 antibody 21579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols