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CYP2F1 Polyclonal antibody

CYP2F1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 21579-1-AP

Print datasheet

Synonyms

C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1

验证数据展示

  • WB analysis of mouse lung using 21579-1-AP

    WB analysis of mouse lung using 21579-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 21579-1-AP (CYP2F1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human lung cancer using 21579-1-AP

    IHC staining of human lung cancer using 21579-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21579-1-AP (CYP2F1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21579-1-AP targets CYP2F1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP2F1 fusion protein Ag16124 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 2, subfamily F, polypeptide 1
Synonyms C2F1, CYP2F, CYP2F1, CYPIIF1, Cytochrome P450 2F1
Calculated Molecular Weight 491 aa, 56 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC109056
Gene Symbol CYP2F1
Gene ID (NCBI) 1572
RRIDAB_10733220
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP24903
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP2F1 antibody 21579-1-APDownload protocol
IHC protocol for CYP2F1 antibody 21579-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols