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CYP2S1 Polyclonal antibody

CYP2S1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13654-1-AP

Print datasheet

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1

验证数据展示

  • WB analysis of mouse lung using 13654-1-AP

    WB analysis of mouse lung using 13654-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 13654-1-AP (CYP2S1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 13654-1-AP

    IHC staining of human stomach cancer using 13654-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 13654-1-AP (CYP2S1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse lung tissue, mouse skin tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13654-1-AP targets CYP2S1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen CYP2S1 fusion protein Ag4320 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 2, subfamily S, polypeptide 1
Synonyms Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight 50-56 kDa
GenBank Accession NumberBC033691
Gene Symbol CYP2S1
Gene ID (NCBI) 29785
RRIDAB_10642148
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP2S1 antibody 13654-1-APDownload protocol
IHC protocol for CYP2S1 antibody 13654-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols