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CYP2S1 Polyclonal antibody

CYP2S1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19100-1-AP

Print datasheet

Synonyms

Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1

验证数据展示

  • WB analysis of COLO 320 using 19100-1-AP

    WB analysis of COLO 320 using 19100-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 19100-1-AP (CYP2S1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovarian cancer using 19100-1-AP

    IHC staining of human ovarian cancer using 19100-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 19100-1-AP (CYP2S1 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inCOLO 320 cells, mouse kidney tissue
Positive IHC detected inhuman ovarian cancer
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:300-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

19100-1-AP targets CYP2S1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen CYP2S1 fusion protein Ag4605 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 2, subfamily S, polypeptide 1
Synonyms Hydroperoxy icosatetraenoate dehydratase, EC:5.3.99.5, EC:4.2.1.152, EC:1.14.14.-, Cytochrome P450 2S1
Calculated Molecular Weight 504 aa, 56 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC033691
Gene Symbol CYP2S1
Gene ID (NCBI) 29785
RRIDAB_10640528
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96SQ9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP2S1 antibody 19100-1-APDownload protocol
IHC protocol for CYP2S1 antibody 19100-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Pharmacol Rep

Expression of CYP2S1 and CYP2W1 in breast cancer epithelial cells and modulation of their expression by synthetic methoxy stilbenes.

Authors - Hanna Szaefer
View Article