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CYP3A5 Polyclonal antibody

CYP3A5 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 13737-1-AP

Print datasheet

Synonyms

CP35, CYP3A5, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3, P450PCN3, PCN3

验证数据展示

  • WB analysis of mouse liver using 13737-1-AP

    WB analysis of mouse liver using 13737-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 13737-1-AP (CYP3A5 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 13737-1-AP

    IHC staining of human liver cancer using 13737-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver cancer using 13737-1-AP

    IHC staining of human liver cancer using 13737-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13737-1-AP (CYP3A5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inmouse liver tissue, human liver tissue, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13737-1-AP targets CYP3A5 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen CYP3A5 fusion protein Ag4674 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 3, subfamily A, polypeptide 5
Synonyms CP35, CYP3A5, CYPIIIA5, Cytochrome P450 3A5, Cytochrome P450 HLp2, Cytochrome P450 PCN3, P450PCN3, PCN3
Calculated Molecular Weight 502 aa, 57 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC033862
Gene Symbol CYP3A5
Gene ID (NCBI) 1577
RRIDAB_2090516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP20815
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for CYP3A5 antibody 13737-1-APDownload protocol
IHC protocol for CYP3A5 antibody 13737-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC

Front Oncol

Novel Prognostic Signatures of Hepatocellular Carcinoma Based on Metabolic Pathway Phenotypes.

Authors - Tingbo Ye
View Article

Oncotarget

Alpha-ketoglutarate suppresses the NF-κB-mediated inflammatory pathway and enhances the PXR-regulated detoxification pathway.

Authors - Liuqin He
View Article
humanWB

Int J Mol Sci

Gene Expression Reprogramming by Citrate Supplementation Reduces HepG2 Cell Migration and Invasion

Authors - Rocchina Miglionico
View Article