• 概述
  • 经过测试的应用
  • 产品信息
  • 发表文章 (14)
  • 实验方案

CYP46A1 Polyclonal antibody

CYP46A1 Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, IP, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 12486-1-AP

Print datasheet

Synonyms

CP46, Cholesterol 24S-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24-hydroxylase, Cholesterol 24 hydroxylase



经过测试的应用

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12486-1-AP targets CYP46A1 in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, IP, ELISA Application Description
Cited ApplicationsWB, IHC, IF
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Immunogen CYP46A1 fusion protein Ag3116 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 46, subfamily A, polypeptide 1
Synonyms CP46, Cholesterol 24S-hydroxylase, Cholesterol 24-monooxygenase, Cholesterol 24-hydroxylase, Cholesterol 24 hydroxylase
Calculated Molecular Weight 500 aa, 57 kDa
Observed Molecular Weight 57 kDa
GenBank Accession NumberBC022539
Gene Symbol CYP46A1
Gene ID (NCBI) 10858
RRIDAB_2090661
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y6A2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cytochrome P450 46A1 (CYP46A1), also named as CYP46, P450 46A1, CH24H and Cholesterol 24-hydroxylase, belongs to the cytochrome P450 family. CYP46A1 is a central nervous system-specific enzyme, and can freely cross the blood-brain barrier and be degraded in the liver. It is involved in the turnover of cholesterol. It converts cholesterol into 24S-hydroxycholesterol and, to a lesser extent, 25-hydroxycholesterol. CYP46A1 is significantly upregulated in hepatic lymphomyeloid cells compared with ESCs.(PMID:20039312)

实验方案

Product Specific Protocols
WB protocol for CYP46A1 antibody 12486-1-APDownload protocol
IHC protocol for CYP46A1 antibody 12486-1-APDownload protocol
IF protocol for CYP46A1 antibody 12486-1-APDownload protocol
IP protocol for CYP46A1 antibody 12486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

  • Journal Impact Factor
  • Most recent
SpeciesApplicationTitle
human,mouseWB,IHC

Cell Rep Med

Cholesterol 24-hydroxylase at the choroid plexus contributes to brain immune homeostasis

Authors - Afroditi Tsitsou-Kampeli
mouseIF

Nat Commun

ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.

Authors - Yuanyuan Zhao
mouseWB

EMBO Rep

NPC1 enables cholesterol mobilization during long-term potentiation that can be restored in Niemann-Pick disease type C by CYP46A1 activation.

Authors - Daniel N Mitroi
mouseWB

Oncogene

Isocitrate dehydrogenase 1 mutation enhances 24(S)-hydroxycholesterol production and alters cholesterol homeostasis in glioma.

Authors - Risheng Yang
humanWB

Neurobiol Aging

H63D mutation in hemochromatosis alters cholesterol metabolism and induces memory impairment.

Authors - Fatima Ali-Rahmani
ratIF

Sci Rep

24(S)-Hydroxycholesterol protects the ex vivo rat retina from injury by elevated hydrostatic pressure.

Authors - Makoto Ishikawa