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  • Featured Product
  • KD/KO Validated

CYP4F2-Specific Polyclonal antibody, PBS Only

CYP4F2-Specific Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 20010-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis using 20010-1-AP (same clone as 20010-1-PBS)

    WB analysis using 20010-1-AP (same clone as 20010-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

  • WB analysis of A549 using 20010-1-AP (same clone as 20010-1-PBS)

    WB analysis of A549 using 20010-1-AP (same clone as 20010-1-PBS)

    WB result of CYP4F2-Specific antibody (20010-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CYP4F2-Specific transfected A549 cells. This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

    IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

    IHC staining of human liver using 20010-1-AP (same clone as 20010-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 20010-1-AP (CYP4F2-Specific antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20010-1-PBS in a different storage buffer formulation.

产品信息

20010-1-PBS targets CYP4F2-Specific in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 4, subfamily F, polypeptide 2
Synonyms
Calculated Molecular Weight 60 kDa
Observed Molecular Weight 50-53 kDa
GenBank Accession NumberNM_001082
Gene Symbol CYP4F2
Gene ID (NCBI) 8529
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78329
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP4F2, also naemed as CPF2, belongs to the cytochrome P450 family. Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, CYP4F2 is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This antibody is specific to CYP4F2 with an observed molecular weight of 50-53 kDa (PMID: 17341693, PMID: 37121548) . It has no cross reaction to other CYP4F member.