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CYP7A1 Polyclonal antibody, PBS Only

CYP7A1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 18054-1-PBS

Print datasheet

Synonyms

Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase

验证数据展示

  • WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

    WB analysis using 18054-1-AP (same clone as 18054-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 18054-1-AP (CYP7A1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    IHC staining of human liver using 18054-1-AP (same clone as 18054-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18054-1-AP (CYP7A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 18054-1-AP (same clone as 18054-1-PBS)

    IF Staining of HepG2 using 18054-1-AP (same clone as 18054-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CYP7A1 antibody (18054-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 18054-1-PBS in a different storage buffer formulation.

产品信息

18054-1-PBS targets CYP7A1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human
Immunogen CYP7A1 fusion protein Ag12572 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cytochrome P450, family 7, subfamily A, polypeptide 1
Synonyms Cytochrome P450 7A1, CYPVII, CYP7, CP7A, Cholesterol 7-alpha-monooxygenase
Calculated Molecular Weight 504 aa, 58 kDa
Observed Molecular Weight 58 kDa
GenBank Accession NumberBC101777
Gene Symbol CYP7A1
Gene ID (NCBI) 1581
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP22680
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CYP7A1 (Cytochrome P450 Family 7 Subfamily A Member 1), is a cholesterol 7alpha-hydroxylase, catalyzing the first and rate-limiting step in the neutral or classic pathway for bile acid biosynthesis. CYP7A1 is a postulated gene modifier of colorectal cancer risk and target for the therapeutic bile acid, ursodeoxycholic acid (UDCA). The CYP7A1 enzyme controls the rate-limiting step of the catabolism of cholesterol into bile acids in the liver.