验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HEK-293 cells, mouse liver tissue |
| Positive IHC detected in | human liver cancer tissue, human brain tissue, human liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HEK-293 cells, HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy CYP7B1 Ready-To-Use IHC Kit. CYP7B1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 26 publications below |
| IHC | See 2 publications below |
产品信息
24889-1-AP targets CYP7B1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | CYP7B1 fusion protein Ag19374 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cytochrome P450, family 7, subfamily B, polypeptide 1 |
| 别名 | Cytochrome P450 7B1, CP7B, CBAS3, 3-hydroxysteroid 7-alpha hydroxylase, 25/26-hydroxycholesterol 7-alpha-hydroxylase |
| 计算分子量 | 506 aa, 58 kDa |
| 观测分子量 | 50-55 kDa |
| GenBank蛋白编号 | BC136574 |
| 基因名称 | CYP7B1 |
| Gene ID (NCBI) | 9420 |
| RRID | AB_2879780 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O75881 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CYP7B1(Cytochrome P450 7B1) is also named as 25-hydroxycholesterol 7-alpha-hydroxylase and belongs to the cytochrome P450 family. It catalyzes the first and rate-limiting step in the coonversion of cholesterol to bile acids and CYP7B1-mediated catalysis may play a role for control of the cellular levels of androgens, not only of estrogens.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| IHC protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| IF protocol for CYP7B1 antibody 24889-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Free Radic Biol Med ALDH2 deficiency exacerbates MCD-diet induced MASLD by modulating bile acid metabolism | ||
Cell Physiol Biochem Ganoderma Lucidum Polysaccharide Peptide Alleviates Hepatoteatosis via Modulating Bile Acid Metabolism Dependent on FXR-SHP/FGF. | ||
Mol Nutr Food Res The Effect of Coenzyme Q10 Supplementation on Bile Acid Metabolism: Insights from Network Pharmacology, Molecular Docking, and Experimental Validation | ||
J Nutr Biochem Deficiency of coiled-coil domain containing 80 increases plasma cholesterol by decreasing fecal sterols excretion in hypercholesterolemic mice. | ||
Biochem Pharmacol Association between the perturbation of bile acid homeostasis and valproic acid-induced hepatotoxicity. | ||
J Lipid Res Insulin resistance dysregulates CYP7B1 leading to oxysterol accumulation: a pathway for NAFL to NASH transition. |
