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CoraLite® Plus 488-conjugated CYR61 Monoclonal antibody

CYR61 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E11F11

Cat no : CL488-67656

Print datasheet

Synonyms

CYR61/CCN1, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1, CCN family member 1

验证数据展示

  • IF Staining of MCF-7 using CL488-67656

    IF Staining of MCF-7 using CL488-67656

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 CYR61 antibody (CL488-67656, Clone: 2E11F11 ) at dilution of 1:200.

  • IF Staining of HeLa using CL488-67656

    IF Staining of HeLa using CL488-67656

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 CYR61 antibody (CL488-67656, Clone: 2E11F11 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy CYR61 Ready-To-Use IHC Kit. CYR61 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67656-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67656 targets CYR61 in IF/ICC applications and shows reactivity with human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human
Immunogen CYR61 fusion protein Ag25129 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name cysteine-rich, angiogenic inducer, 61
Synonyms CYR61/CCN1, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1, CCN family member 1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 40-42 kDa
GenBank Accession NumberBC001271
Gene Symbol CYR61
Gene ID (NCBI) 3491
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00622
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 CYR61 antibody CL488-67656Download protocol
Standard Protocols
Click here to view our Standard Protocols