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CYR61/CCN1 Polyclonal antibody

CYR61/CCN1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IF/ICC, ELISA and More (2)

Conjugate

Unconjugated

Cat no : 26689-1-AP

Print datasheet

Synonyms

CYR61, cysteine-rich protein 61, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1



经过测试的应用

Positive WB detected inA431 cells, MDA-MB-453s cells, HeLa cells
Positive IHC detected inhuman colon cancer tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy CYR61 Ready-To-Use IHC Kit. CYR61 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26689-1-AP targets CYR61/CCN1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB, IHC, IF, IP
Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Immunogen CYR61/CCN1 fusion protein Ag25129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name cysteine-rich, angiogenic inducer, 61
Synonyms CYR61, cysteine-rich protein 61, Cysteine-rich angiogenic inducer 61, Cellular communication network factor 1, CCN1
Calculated Molecular Weight 42 kDa
Observed Molecular Weight 42 kDa
GenBank Accession NumberBC001271
Gene Symbol CYR61
Gene ID (NCBI) 3491
RRIDAB_2880604
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00622
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CYR61 is a member of the CYR61/CTGF/NOV (CCN) protein family. CYR61 participated in cell mitogenesis, cellular adhesion, migration, differentiation, angiogenesis, and survival. Cyr61 is especially associated with diseases related to chronic inflammation, including RA, Crohn's disease and ulcerative colitis. CYR61 may serve essential roles as either an oncogene or a tumor suppressor, depending on the cancer cell type. CYR61 also induces angiogenesis, which supplies oxygen and nutrients to tumors during growth. CYR61 can also play a role in the proliferation, invasion, survival, and metastasis of cancer cells.

实验方案

Product Specific Protocols
WB protocol for CYR61/CCN1 antibody 26689-1-APDownload protocol
IHC protocol for CYR61/CCN1 antibody 26689-1-APDownload protocol
IF protocol for CYR61/CCN1 antibody 26689-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB,IF

Nat Commun

A translational program that suppresses metabolism to shield the genome.

Authors - Nathan C Balukoff

ACS Appl Mater Interfaces

A Novel Electrochemiluminescent Immunoassay Based on Target Transformation Assisted with Catalyzed Hairpin Assembly Amplification for the Ultrasensitive Bioassay.

Authors - Hu-Xin Lei
humanWB

Mol Ther Nucleic Acids

The RNA binding protein QKI5 suppresses ovarian cancer via downregulating transcriptional coactivator TAZ.

Authors - Tao Liu
humanWB

Cell Death Dis

EGFR-PI3K-PDK1 pathway regulates YAP signaling in hepatocellular carcinoma: the mechanism and its implications in targeted therapy.

Authors - Hongwei Xia
humanWB,IHC

Cancers (Basel)

WISP2/CCN5 Suppresses Vasculogenic Mimicry through Inhibition of YAP/TAZ Signaling in Breast Cancer Cells.

Authors - Nathalie Ferrand
human,mouseWB

Hum Mol Genet

Abnormal activation of yap/Taz contributes to the pathogenesis of tuberous sclerosis complex.

Authors - Bethany K Terry