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验证数据展示

  • IHC staining of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    IHC staining of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12666-2-AP (CaMKII Gamma antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAMK2G antibody included
  • IHC staining of human pancreas cancer using 12666-2-AP (same clone as 12666-2-PBS)

    IHC staining of human pancreas cancer using 12666-2-AP (same clone as 12666-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 12666-2-AP (CaMKII gamma antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CAMK2G antibody included
  • WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

    WB analysis of SMMC-7721 using 12666-2-AP (same clone as 12666-2-PBS)

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 12666-2-AP (CaMKII gamma antibody at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • IP experiment of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    IP experiment of mouse heart using 12666-2-AP (same clone as 12666-2-PBS)

    IP result of anti-CaMKII Gamma (IP:12666-2-AP, 4ug; Detection:12666-2-AP 1:1000) with mouse heart tissue lysate 3200ug. This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 12666-2-AP (same clone as 12666-2-PBS)

    IF Staining of HepG2 using 12666-2-AP (same clone as 12666-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CaMKII Gamma antibody (12666-2-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 12666-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CaMKII Gamma Polyclonal antibody, PBS Only

CaMKII Gamma Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 12666-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy CAMK2G Ready-To-Use IHC Kit. CAMK2G primary antibody included.

产品信息

12666-2-PBS targets CaMKII Gamma in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CaMKII Gamma fusion protein Ag3348 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 calcium/calmodulin-dependent protein kinase II gamma
别名 CAMK2G, CaMK-II subunit gamma, CAMK-II, CAMK, CaM kinase II subunit gamma
计算分子量 527 aa, 59 kDa
观测分子量 59 kDa
GenBank蛋白编号BC034044
基因名称 CAMK2G
Gene ID (NCBI) 818
RRIDAB_2068213
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ13555
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CaMKII gamma, also named CAMKG or CAMK2G, belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, and CaMK subfamily. CAMK2 is a prominent kinase in the central nervous system that may function in long-term potentiation and neurotransmitter release. CAMK2G is expressed in human beta cells as a candidate gene for Type II (non-insulin-dependent) diabetes mellitus. Some studies can be used to assess the role of CAMK2G in the susceptibility to Type II diabetes(PMID: 12032636). CAMK2G has some isoforms with the MW of 55-66 kDa and this antibody can recognize all the isoforms of CAMK2G. This antibody may have cross-reaction with CAMK2A/B/D due to the high homology.