验证数据展示
经过测试的应用
| Positive WB detected in | rat kidney tissue, mouse kidney tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
27703-1-AP targets Cadherin-6 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | Human, Mouse, Rat |
| 免疫原 | Cadherin-6 fusion protein Ag26756 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | cadherin 6, type 2, K-cadherin (fetal kidney) |
| 别名 | Cadherin 6, CDH6, K cadherin, KCAD, Kidney cadherin |
| 计算分子量 | 790 aa, 88 kDa |
| 观测分子量 | 130 kDa |
| GenBank蛋白编号 | BC000019 |
| 基因名称 | Cadherin-6 |
| Gene ID (NCBI) | 1004 |
| RRID | AB_3085988 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P55285 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Cadherin-6 (CDH6), also known as K-cadherin, is a class II cadherin involved in the morphogenesis of the central nervous system and kidney (PMID: 27375021). Expression of cadherin-6 has also been described in some cancers, including renal cancer, ovarian cancer, gastric cancer, and thyroid cancer (PMID: 14665853; 28526733; 34530820). Cadherin-6 is involved in epithelial-mesenchymal transition (EMT) and cancer metastasis (PMID: 27375021).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Cadherin-6 antibody 27703-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |