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Calbindin-D28k Monoclonal antibody, PBS Only

Calbindin-D28k Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

1F8B9

Cat no : 66394-1-PBS

Print datasheet

Synonyms

Calbindin, CALB1, Calbindin D28, calbindin 1, 28kDa, CALB

验证数据展示

  • WB analysis using 66394-1-Ig (same clone as 66394-1-PBS)

    WB analysis using 66394-1-Ig (same clone as 66394-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    IF Staining of mouse cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse cerebellum tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

  • IHC staining of rat cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    IHC staining of rat cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IHC staining of rat cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    IHC staining of rat cerebellum using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue slide using 66394-1-Ig (Calbindin-D28k antibody) at dilution of 1:16000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calbindin-D28k antibody included
  • IF Staining of human kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    IF Staining of human kidney using 66394-1-Ig (same clone as 66394-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Calbindin-D28k antibody (66394-1-Ig, Clone: 1F8B9 ) at dilution of 1:300 and CoraLite®594-Conjugated Goat Anti-Mouse IgG(H+L), Podocalyxin antibody(18150-1-AP, green). This data was developed using the same antibody clone with 66394-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Calbindin-D28k Ready-To-Use IHC Kit. Calbindin-D28k primary antibody included.

产品信息

66394-1-PBS targets Calbindin-D28k in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

Tested Applications WB, IHC, IF-P, ELISA Application Description
Tested Reactivity human, mouse, rat, pig, rabbit
Immunogen Calbindin-D28k fusion protein Ag5861 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 1, 28kDa
Synonyms Calbindin, CALB1, Calbindin D28, calbindin 1, 28kDa, CALB
Calculated Molecular Weight 30 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC006478
Gene Symbol Calbindin
Gene ID (NCBI) 793
RRIDAB_2881769
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP05937
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calbindin-D28k is a member of the calcium-binding protein superfamily that includes calmodulin and troponin C. It is a cytosolic calcium binding protein highly expressed in the distal tubule, intestines, central nervous system, primary murine osteoblast cells and in several other organs. It plays an important role in the intracellular calcium homeostasis, its strong buffering capacity prevents cytotoxic effect of high concentration of free calcium in kidney, brain, pancreas, and intestine.