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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated Caldesmon Polyclonal antibody

Caldesmon Polyclonal Antibody for IF/ICC, IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-20887

Print datasheet

Synonyms

CALD1, CDM, caldesmon 1, CAD

验证数据展示

  • IF Staining of mouse heart using CL488-20887

    IF Staining of mouse heart using CL488-20887

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse heart tissue using CoraLite® Plus 488 Caldesmon antibody (CL488-20887) at dilution of 1:200. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using CL488-20887

    IF Staining of HeLa using CL488-20887

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 Caldesmon antibody (CL488-20887) at dilution of 1:200, CL594-phalloidin (red).

经过测试的应用

Positive IF-P detected inmouse heart tissue
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALD1 Ready-To-Use IHC Kit. CALD1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 20887-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-20887 targets Caldesmon in IF/ICC, IF-P applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC, IF-P Application Description
Tested Reactivity human, mouse
Immunogen Caldesmon fusion protein Ag13676 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caldesmon 1
Synonyms CALD1, CDM, caldesmon 1, CAD
Calculated Molecular Weight 538 aa, 63 kDa
Observed Molecular Weight 70-80 kDa
GenBank Accession NumberBC040354
Gene Symbol CALD1
Gene ID (NCBI) 800
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05682
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caldesmon (CALD1), a protein found in the cytoskeleton, plays an important role in proliferation, apoptosis, cell motility, adhesion, receptor function, and second messenger pathways. Caldesmon is a substrate of cdc2 kinase and Erk1/2 MAPK, and phosphorylation by either of these kinases reverses the inhibitory effects of caldesmon. Caldesmon(CALD1) is a prognostic biomarker and correlated with bladder cancer(PMID: 34733993) and immune infiltrates in gastric cancers(PMID: 34189308).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Caldesmon antibody CL488-20887Download protocol
Standard Protocols
Click here to view our Standard Protocols