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验证数据展示

  • WB analysis using 66540-1-Ig

    WB analysis using 66540-1-Ig

    Various tissue were subjected to SDS PAGE followed by western blot with 66540-1-Ig (Calponin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human myofibroblastoma using 66540-1-Ig

    IHC staining of human myofibroblastoma using 66540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human myofibroblastoma using 66540-1-Ig

    IHC staining of human myofibroblastoma using 66540-1-Ig

    Immunohistochemical analysis of paraffin-embedded human myofibroblastoma tissue slide using 66540-1-Ig (Calponin antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Calponin Monoclonal antibody

Calponin Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66540-1-Ig

产品说明书

CloneNo. 1A11E5
引用文献(1)

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhuman heart tissue, human vein tissue, human colon tissue, human ileum tissue, human bladder tissue, human kidney tissue
Positive IHC detected inhuman myofibroblastoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1500-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66540-1-Ig targets Calponin in WB, IHC, IF, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human
文献引用反应性rat
免疫原 Calponin fusion protein Ag21384 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 calponin 1, basic, smooth muscle
别名 Basic calponin, Calponin, Calponin 1, Calponin H1, smooth muscle, CNN1, Sm Calp, SMCC
计算分子量 33 kDa
观测分子量 33-35 kDa
GenBank蛋白编号BC022015
基因名称 Calponin
Gene ID (NCBI) 1264
RRIDAB_2881902
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP51911
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). CNN1 is a basic 34-kD protein specifically expressed in smooth muscle and a marker of smooth muscle cell differentiation. This antibody raised against the specific region of human CNN1, thus it does not cross-react with CNN2 or CNN3.

实验方案

Product Specific Protocols
WB protocol for Calponin antibody 66540-1-IgDownload protocol
IHC protocol for Calponin antibody 66540-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
ratWB,IF

World J Stem Cells

Bone marrow mesenchymal stem cells promote uterine healing by activating the PI3K/AKT pathway and modulating inflammation in rat models

Authors - Jing Yang
View Article