WB Figures
WB analysis of mouse brain using 66496-1-Ig (same clone as 66496-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of mouse cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of pig brain using 66496-1-Ig (same clone as 66496-1-PBS)
pig brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)
rat brain tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of rat cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of U-251 using 66496-1-Ig (same clone as 66496-1-PBS)
U-251 cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
WB analysis of U2OS using 66496-1-Ig (same clone as 66496-1-PBS)
U2OS cells were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:16000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human cerebellum using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of human colon using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IHC staining of rat brain using 66496-1-Ig (same clone as 66496-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF-P Figures
IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)
Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.
CYTOMETRIC BEAD ARRAY Figures
Cytometric bead array standard curve of MP50295-3
Cytometric bead array standard curve of MP50295-3, Calretinin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66496-6-PBS. Detection antibody: 66496-1-PBS. Standard:Ag2924. Range: 3.125-100 ng/mL.
