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Calretinin Monoclonal antibody, PBS Only (Detector)

Calretinin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7A9

Cat no : 66496-1-PBS

Print datasheet

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2D7A9

验证数据展示

  • WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

    WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

    IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50295-3

    Cytometric bead array standard curve of MP50295-3

    Cytometric bead array standard curve of MP50295-3, Calretinin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66496-6-PBS. Detection antibody: 66496-1-PBS. Standard:Ag2924. Range: 3.125-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

产品信息

66496-1-PBS targets Calretinin as part of a matched antibody pair:

MP50295-3: 66496-6-PBS capture and 66496-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms CALB2, calbindin 2, CAL2, CAB29, 2D7A9
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
RRIDAB_2868480
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.