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Calretinin Monoclonal antibody, PBS Only (Detector)

Calretinin Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D7A9

Cat no : 66496-1-PBS

Print datasheet

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2D7A9

验证数据展示

  • WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

    WB analysis using 66496-1-Ig (same clone as 66496-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66496-1-Ig (Calretinin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

    IF Staining of SH-SY5Y using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IHC staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66496-1-Ig (Calretinin antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    IF Staining of human appendicitis using 66496-1-Ig (same clone as 66496-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using Calretinin antibody (66496-1-Ig, Clone: 2D7A9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66496-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP50295-3

    Cytometric bead array standard curve of MP50295-3

    Cytometric bead array standard curve of MP50295-3, Calretinin Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66496-6-PBS. Detection antibody: 66496-1-PBS. Standard:Ag2924. Range: 3.125-100 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

产品信息

66496-1-PBS targets Calretinin as part of a matched antibody pair:

MP50295-3: 66496-6-PBS capture and 66496-1-PBS detection (validated in Cytometric bead array)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, IF/ICC, IF-P, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calbindin 2
Synonyms CALB2, calbindin 2, CAL2, CAB29, 2D7A9
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP22676
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.