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Calretinin Recombinant antibody

Calretinin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F20

Cat no : 82811-1-RR

Print datasheet

Synonyms

CALB2, calbindin 2, CAL2, CAB29, 2F20

验证数据展示

  • IF Staining of adult mouse cerebellum tissue using 82811-1-RR

    IF Staining of adult mouse cerebellum tissue using 82811-1-RR

    Immunofluorescent analysis of (4% PFA) fixed adult mouse cerebellum tissue using Calretinin antibody (82811-1-RR) at dilution of 1:500 and Alexa Fluor 594 Donkey Anti-Rabbit IgG (H+L). (Image produced by Watt Lab at McGill University)

  • WB analysis using 82811-1-RR

    WB analysis using 82811-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82811-1-RR (Calretinin antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82811-1-RR

    IHC staining of mouse brain using 82811-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calretinin antibody included
  • IHC staining of mouse brain using 82811-1-RR

    IHC staining of mouse brain using 82811-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82811-1-RR (Calretinin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Calretinin antibody included
  • IF Staining of SH-SY5Y using 82811-1-RR

    IF Staining of SH-SY5Y using 82811-1-RR

    Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using Calretinin antibody (82811-1-RR, Clone: 2F20 ) at dilution of 1:250 and Multi-rAb CoraLite ® Plus 594-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR004).

  • FC experiment of SH-SY5Y using 82811-1-RR

    FC experiment of SH-SY5Y using 82811-1-RR

    1x10^6 SH-SY5Y cells were intracellularly stained with 0.25 ug Calretinin Recombinant antibody (82811-1-RR, Clone:2F20) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 82811-1-RR

    Affinity and Kinetic Characterization of 82811-1-RR

    Biolayer interferometry (BLl) kinetic assays of 82811-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

经过测试的应用

Positive WB detected inSH-SY5Y cells, U2OS cells, fetal human brain tissue, mouse brain tissue, rat brain tissue, pig brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSH-SY5Y cells
Positive FC (Intra) detected inSH-SY5Y cells
Planning an IHC experiment? We recommend our IHCeasy Calretinin Ready-To-Use IHC Kit. Calretinin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5100
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:125-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82811-1-RR targets Calretinin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen Calretinin fusion protein Ag2924 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name calbindin 2
Synonyms CALB2, calbindin 2, CAL2, CAB29, 2F20
Calculated Molecular Weight 29 kDa
Observed Molecular Weight29 kDa
GenBank Accession NumberBC015484
Gene Symbol Calretinin
Gene ID (NCBI) 794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP22676
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Calbindin 2 (calretinin), is an intracellular calcium-binding protein belonging to the troponin C superfamily. Members of this protein family have six EF-hand domains which bind calcium. This protein plays a role in diverse cellular functions, including message targeting and intracellular calcium buffering. It also functions as a modulator of neuronal excitability, and is a diagnostic marker for some human diseases, including Hirschsprung disease and some cancers. Calretinin is a useful marker for differentiating malignant mesothelioma from carcinomas.

实验方案

Product Specific Protocols
WB protocol for Calretinin antibody 82811-1-RRDownload protocol
IHC protocol for Calretinin antibody 82811-1-RRDownload protocol
IF protocol for Calretinin antibody 82811-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols