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Calsequestrin 2 Monoclonal antibody, PBS Only

Calsequestrin 2 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, pig, mouse

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C10A1

Cat no : 66419-1-PBS

Print datasheet

Synonyms

Calsequestrin 2, CASQ2, PDIB2

验证数据展示

  • WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

    WB analysis using 66419-1-Ig (same clone as 66419-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66419-1-Ig (Calsequestrin 2 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 10x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IHC staining of human heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66419-1-Ig (Calsequestrin 2 antibody at dilution of 1:400 (under 40x lens). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    IF Staining of mouse heart using 66419-1-Ig (same clone as 66419-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using Calsequestrin 2 antibody (66419-1-Ig, Clone: 1C10A1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66419-1-PBS in a different storage buffer formulation.

产品信息

66419-1-PBS targets Calsequestrin 2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, rat, pig, mouse samples.

Tested Applications WB, IHC, IF-P, Indirect ELISA Application Description
Tested Reactivity human, rat, pig, mouse
Immunogen Calsequestrin 2 fusion protein Ag13246 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name calsequestrin 2 (cardiac muscle)
Synonyms Calsequestrin 2, CASQ2, PDIB2
Calculated Molecular Weight 46 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC022288
Gene Symbol Calsequestrin 2
Gene ID (NCBI) 845
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14958
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Calsequestrin (CASQ) is a Ca2+-binding protein present primarily in junctional sarcoplasmic reticulum of skeletal and cardiac muscle; the cardiac form (CASQ2) is encoded by a separate gene. The primary role of CASQ2 is buffering of the sarcoplasmic reticulum Ca2+ ions, but another role for CASQ2 has emerged recently: CASQ2 regulates the open probability of ryanodine receptor 2 (RyR2). Mutations in CASQ2 cause stress-induced polymorphic ventricular tachycardia, also referred to as catecholaminergic polymorphic ventricular tachycardia 2 (CPVT2), a disease characterized by bidirectional ventricular tachycardia that may lead to cardiac arrest.