• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Calumenin Monoclonal antibody

Calumenin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2D8B8

Cat no : 67585-1-Ig

Print datasheet

Synonyms

CALU, calumenin, Crocalbin, FLJ90608, IEF SSP 9302

验证数据展示

  • WB analysis using 67585-1-Ig

    WB analysis using 67585-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67585-1-Ig (CALU antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 67585-1-Ig

    IHC staining of human colon cancer using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CALU antibody included
  • IF Staining of HeLa using 67585-1-Ig

    IF Staining of HeLa using 67585-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Calumenin antibody (67585-1-Ig, Clone: 2D8B8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red).

  • IHC staining of human colon cancer using 67585-1-Ig

    IHC staining of human colon cancer using 67585-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67585-1-Ig (Calumenin antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with CALU antibody included

经过测试的应用

Positive WB detected inHeLa cells, NIH/3T3 cells, HepG2 cells, HSC-T6 cells, Jurkat cells, RAW264.7 cells, HEK-293 cells
Positive IHC detected inhuman colon cancer tissue, human cervical cancer tissue, human kidney tissue, mouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy CALU Ready-To-Use IHC Kit. CALU primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67585-1-Ig targets Calumenin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen Calumenin fusion protein Ag12073 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name calumenin
Synonyms CALU, calumenin, Crocalbin, FLJ90608, IEF SSP 9302
Calculated Molecular Weight 315 aa, 37 kDa
Observed Molecular Weight 47-50 kDa
GenBank Accession NumberBC013383
Gene Symbol Calumenin
Gene ID (NCBI) 813
RRIDAB_2882794
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO43852
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Calumenin antibody 67585-1-IgDownload protocol
IHC protocol for Calumenin antibody 67585-1-IgDownload protocol
IF protocol for Calumenin antibody 67585-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols