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验证数据展示

  • WB analysis of MCF-7 using 29371-1-AP

    WB analysis of MCF-7 using 29371-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 29371-1-AP (Cannabinoid receptor 2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 29371-1-AP

    IHC staining of mouse brain using 29371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29371-1-AP (Cannabinoid receptor 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 29371-1-AP

    IHC staining of mouse brain using 29371-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 29371-1-AP (Cannabinoid receptor 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Cannabinoid receptor 2 Polyclonal antibody

Cannabinoid receptor 2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 29371-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA

别名

CNR2, CB2B, CB2A, CB-2, CB2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

29371-1-AP targets Cannabinoid receptor 2 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原 Cannabinoid receptor 2 fusion protein Ag30609 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cannabinoid receptor 2 (macrophage)
别名 CNR2, CB2B, CB2A, CB-2, CB2
计算分子量 360 aa, 40 kDa
观测分子量60 kDa
GenBank蛋白编号BC074767
基因名称 Cannabinoid receptor 2
Gene ID (NCBI) 1269
RRIDAB_2918285
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP34972
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cannabinoid receptor 1 (CNR1, or CB1) and CNR2 (CB2) are members of the guanine-nucleotide-binding protein (G-protein) coupled receptor family. The CB1 receptor is expressed mainly in the brain. The CB2 receptor is expressed mainly in the immune system and in hematopoietic cells. The two receptors have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana.

实验方案

Product Specific Protocols
WB protocol for Cannabinoid receptor 2 antibody 29371-1-APDownload protocol
IHC protocol for Cannabinoid receptor 2 antibody 29371-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

World J Gastroenterol

Role of gut microbiota in Crohn's disease pathogenesis: Insights from fecal microbiota transplantation in mouse model

Authors - Qiang Wu
View Article