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验证数据展示

  • IF Staining of HepG2 using 13710-1-AP (same clone as 13710-1-PBS)

    IF Staining of HepG2 using 13710-1-AP (same clone as 13710-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    WB analysis of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IF Staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using Carboxypeptidase E antibody (13710-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IP experiment of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IP result of anti-Carboxypeptidase E (IP:13710-1-AP, 3ug; Detection:13710-1-AP 1:500) with mouse brain tissue lysate 3500ug. This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    IHC staining of mouse brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

    IHC staining of human pituitary using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • IHC staining of rat brain using 13710-1-AP (same clone as 13710-1-PBS)

    IHC staining of rat brain using 13710-1-AP (same clone as 13710-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 13710-1-AP (Carboxypeptidase E antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13710-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

CPE Polyclonal antibody, PBS Only

CPE Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA
Cat No. 13710-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA

别名

Carboxypeptidase E, Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, Carboxypeptidase H

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

13710-1-PBS targets CPE in WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IF-P, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 CPE fusion protein Ag4647 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 carboxypeptidase E
别名 Carboxypeptidase E, Prohormone-processing carboxypeptidase, EC:3.4.17.10, CPH, Carboxypeptidase H
计算分子量 476 aa, 53 kDa
观测分子量 50 kDa, 52 kDa
GenBank蛋白编号BC033866
基因名称 Carboxypeptidase E
Gene ID (NCBI) 1363
RRIDAB_2083913
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP16870
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CPE(Carboxypeptidase E) is also named as CPH.It belongs to the peptidase M14 family and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin.The deduced 476-amino acid human protein contains an N-terminal signal peptide, followed by a 'pro' sequence, a polyarginine stretch, and 2 potential N-glycosylation sites.