验证数据展示
经过测试的应用
| Positive WB detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 4 publications below |
| IHC | See 1 publications below |
产品信息
31020-1-AP targets Caspase 1 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.
| Tested Applications | WB, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | mouse, rat |
| Cited Reactivity | mouse, rat |
| Immunogen | Caspase 1 fusion protein Ag34441 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | caspase 1 |
| Synonyms | Casp1, m CASP1, mouse CASP1, mouse caspase 1, CASP 1 |
| Observed Molecular Weight | 35-40 kDa |
| GenBank Accession Number | BC008152 |
| Gene Symbol | Caspase 1 |
| Gene ID (NCBI) | 12362 |
| RRID | AB_3669817 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | P29452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
CASP1(caspase-1) is also named as IL1BC, IL1BCE and belongs to the peptidase C14A family. It is a cysteine protease that regulates inflammatory processes through its capacity to process and activate the interleukin-1-beta (IL1B), IL18, and IL33 precursor proteins. The active caspase-1 can increase cellular membrane permeability and intracellular calcium levels, which facilitates lysosome exocytosis and release of host antimicrobial factors and microbial products (PMID:21804020). 31020-1-AP is designed for mouse caspase 1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for Caspase 1 antibody 31020-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol Molecular mechanism of Rolupram reducing neuroinflammation in noise induced tinnitus mice through TLR4/NF kB/NLRP3 protein/caspase-1/IL-1 β signaling pathway | ||
Ecotoxicol Environ Saf Copper induced cytosolic escape of mitochondrial DNA and activation of cGAS-STING-NLRP3 pathway-dependent pyroptosis in C8-D1A cells | ||
Phytomedicine Paeoniflorin inhibited GSDMD to alleviate ANIT-induced cholestasis via pyroptosis signaling pathway | ||
Heliyon High-intensity interval training and moderate-intensity continuous training alleviate vascular dysfunction in spontaneously hypertensive rats through the inhibition of pyroptosis | ||
J Inflamm Res The Onset of Systemic Lupus Erythematosus Triggers Nucleus Pulposus Cell Pyroptosis to Exacerbate Intervertebral Disc Degeneration |