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Caspase 3 Recombinant antibody

Caspase 3 Recombinant Antibody for FC, IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC, IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

5G20

Cat No : 82202-1-RR

Print datasheet

Synonyms

Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1

验证数据展示

  • WB analysis of Jurkat using 82202-1-RR

    WB analysis of Jurkat using 82202-1-RR

    Staurosporine treated Jurkat cells were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 82202-1-RR

    WB analysis using 82202-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82202-1-RR (Caspase 3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 82202-1-RR

    IHC staining of mouse brain using 82202-1-RR

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82202-1-RR (Caspase 3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 82202-1-RR

    IF Staining of HeLa using 82202-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • IF Staining of HeLa using 82202-1-RR

    IF Staining of HeLa using 82202-1-RR

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Caspase 3 antibody (82202-1-RR, Clone: 5G20 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 82202-1-RR

    FC experiment of HepG2 using 82202-1-RR

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Caspase 3 (82202-1-RR, Clone:5G20) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inStaurosporine treated Jurkat cells, HepG2 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:500-1:2000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

82202-1-RR targets Caspase 3 in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, IF/ICC, IHC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name caspase 3, apoptosis-related cysteine peptidase
Synonyms Apopain, CASP 3, CASP3, Caspase 3, Caspase3, CPP 32, CPP32, CPP32B, Cysteine protease CPP32, Protein Yama, SCA 1, SREBP cleavage activity 1
Calculated Molecular Weight 32 kDa
Observed Molecular Weight32-35 kDa, 17 kDa, 19 kDa
GenBank Accession NumberNM_004346
Gene Symbol CASP3
Gene ID (NCBI) 836
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP42574
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 (also named CPP32, SCA-1, and Apopain) proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at the beginning of apoptosis. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can also form heterocomplex with other proteins and performs the molecular mass of 50-70 kDa. This antibody can recognize p17, p19 and p32 of Caspase 3.

实验方案

Product Specific Protocols
WB protocol for Caspase 3 antibody 82202-1-RRDownload protocol
IHC protocol for Caspase 3 antibody 82202-1-RRDownload protocol
IF protocol for Caspase 3 antibody 82202-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols