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  • Featured Product
  • KD/KO Validated

Cathepsin D Monoclonal antibody, PBS Only

Cathepsin D Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2F6F7

Cat no : 66534-1-PBS

Print datasheet

Synonyms

CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain

验证数据展示

  • WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

    WB analysis using 66534-1-Ig (same clone as 66534-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66534-1-Ig (Cathepsin D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    IHC staining of human liver cancer using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66534-1-Ig (Cathepsin D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with Cathepsin D antibody included
  • IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    IF Staining of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Cathepsin D antibody (66534-1-Ig, Clone: 2F6F7 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    FC experiment of HepG2 using 66534-1-Ig (same clone as 66534-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human Cathepsin D (66534-1-Ig, Clone:2F6F7) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66534-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Cathepsin D Ready-To-Use IHC Kit. Cathepsin D primary antibody included.

产品信息

66534-1-PBS targets Cathepsin D in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human
Immunogen Cathepsin D fusion protein Ag15254 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name cathepsin D
Synonyms CTSD, CPSD, CLN10, Cathepsin D light chain, Cathepsin D heavy chain
Calculated Molecular Weight 412 aa, 45 kDa
Observed Molecular Weight 32 kDa, 48 kDa, 52 kDa
GenBank Accession NumberBC016320
Gene Symbol Cathepsin D
Gene ID (NCBI) 1509
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07339
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

CTSD (Cathepsin D) is also named as CPSD, belongs to the peptidase A1 family. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Human CTSD is synthesized as a 52-kDa precursor that is converted into an active 48-kDa single-chain intermediate in the endosomes, and then into a fully active mature form, composed of a 34-kDa heavy chain and a 14-kDa light chain, in the lysosomes. It is a lysosomal acid protease found in neutrophils and monocytes and involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease. (PMID: 27114232, PMID: 30717773, PMID: 30051532)