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Caveolin-3 Polyclonal antibody

Caveolin-3 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 28358-1-AP

Print datasheet

Synonyms

CAV3, Caveolin3, M-caveolin, LQT9, LGMD1C

验证数据展示

  • WB analysis of mouse colon using 28358-1-AP

    WB analysis of mouse colon using 28358-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 28358-1-AP (Caveolin-3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse heart using 28358-1-AP

    IHC staining of mouse heart using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-3 antibody included
  • IHC staining of mouse heart using 28358-1-AP

    IHC staining of mouse heart using 28358-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 28358-1-AP (Caveolin-3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Caveolin-3 antibody included
  • IF Staining of mouse skeletal muscle using 28358-1-AP

    IF Staining of mouse skeletal muscle using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of mouse skeletal muscle using 28358-1-AP

    IF Staining of mouse skeletal muscle using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using Caveolin-3 antibody (28358-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of H9C2 using 28358-1-AP

    IF Staining of H9C2 using 28358-1-AP

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using Caveolin-3 antibody (28358-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

经过测试的应用

Positive WB detected inmouse colon tissue, mouse heart tissue, mouse skeletal muscle tissue
Positive IHC detected inmouse heart tissue, rat skeletal muscle tissue, mouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse skeletal muscle tissue
Positive IF/ICC detected inH9C2 cells
Planning an IHC experiment? We recommend our IHCeasy Caveolin-3 Ready-To-Use IHC Kit. Caveolin-3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28358-1-AP targets Caveolin-3 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IF-P, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen Caveolin-3 fusion protein Ag28009 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name caveolin 3
Synonyms CAV3, Caveolin3, M-caveolin, LQT9, LGMD1C
Calculated Molecular Weight 151 aa, 17 kDa
Observed Molecular Weight 20 kDa
GenBank Accession NumberBC069368
Gene Symbol Caveolin-3
Gene ID (NCBI) 859
RRIDAB_2881120
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP56539
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Caveolin-3 antibody 28358-1-APDownload protocol
IHC protocol for Caveolin-3 antibody 28358-1-APDownload protocol
IF protocol for Caveolin-3 antibody 28358-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,mouseWB

Oxid Med Cell Longev

Trophoblasts Modulate the Ca2+ Oscillation and Contraction of Myometrial Smooth Muscle Cells by Small Extracellular Vesicle- (sEV-) Mediated Exporting of miR-25-3p during Premature Labor.

Authors - Lin Wang
View Article