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验证数据展示

  • WB analysis using 31520-1-AP

    WB analysis using 31520-1-AP

    Untreated, LPS and Brefeldin A treated RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 31520-1-AP (Ccl2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

MCP-1/CCL2 Polyclonal antibody

MCP-1/CCL2 Polyclonal Antibody for WB, ELISA
Cat No. 31520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, ELISA

别名

Ccl2, Chemokine C C motif ligand 2, chemokine (C C motif) ligand 2, CCL-2, CCL 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLPS and Protein transport inhibitor treated RAW 264.7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31520-1-AP targets MCP-1/CCL2 in WB, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 mouse, rat
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 chemokine (C-C motif) ligand 2
别名 Ccl2, Chemokine C C motif ligand 2, chemokine (C C motif) ligand 2, CCL-2, CCL 2
计算分子量16 kDa
观测分子量20 kDa
GenBank蛋白编号NM_031530
基因名称 MCP-1/CCL2
Gene ID (NCBI) 24770
RRIDAB_3670017
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDP14844
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Monocyte chemoattractant protein-1 (MCP-1/CCL2) is one of the key chemokines that regulate migration and infiltration of monocytes/macrophages. Both CCL2 and its receptor CCR2 have been demonstrated to be induced and involved in various diseases. Migration of monocytes from the blood stream across the vascular endothelium is required for routine immunological surveillance of tissues, as well as in response to inflammation.

实验方案

Product Specific Protocols
WB protocol for MCP-1/CCL2 antibody 31520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols