p21 Polyclonal antibody

p21 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 28248-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

mouse, rat

应用

WB, IF/ICC, IP, ELISA and More (3)

Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inPC-12 cells, NIH/3T3 cells, DC2.4 cells, HSC-T6 cells, RAW 264.7 cells, Sp2/0 cells, Neuro-2a cells
Positive IP detected inNIH/3T3 cells
Positive IF/ICC detected inNIH/3T3 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28248-1-AP targets p21 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with mouse, rat samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF, IP, CoIP
经测试反应性 mouse, rat
文献引用反应性mouse, rat
免疫原 p21 fusion protein Ag28394 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 cyclin-dependent kinase inhibitor 1A (P21)
别名 Cdkn1a, p21WAF, p21Cip1, P21 protein, Cdkn1
计算分子量 18 kDa
观测分子量 18 kDa
GenBank蛋白编号NM_001111099
基因名称 p21
Gene ID (NCBI) 12575
RRIDAB_2881097
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP39689
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

CDKN1A (p21, CIP1, WAF1) is a cyclin-dependent kinase inhibitor. CDKN1A binds to and inhibits the activity of cyclin-CDK2 or -CDK4 complexes, and thus functions as a regulator of cell cycle progression at the G1 phase. The expression of CDKN1A is induced by wild-type but not mutant p53 protein, through which CDKN1A mediates the p53-dependent cell cycle G1 phase arrest in response to a variety of stress stimuli. CDKN1A can interact with proliferating cell nuclear antigen (PCNA), and plays a regulatory role in S phase DNA replication and DNA damage repair. CDKN1A was reported to be specifically cleaved by CASP3-like caspases, which thus leads to a dramatic activation of CDK2, and may be instrumental in the execution of apoptosis following caspase activation.

实验方案

Product Specific Protocols
WB protocol for p21 antibody 28248-1-APDownload protocol
IF protocol for p21 antibody 28248-1-APDownload protocol
IP protocol for p21 antibody 28248-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bioact Mater

Chemo-immunotherapy by dual-enzyme responsive peptide self-assembling abolish melanoma

Authors - Yuhan Wang
mouseWB

Redox Biol

Deficiency of S100 calcium binding protein A9 attenuates vascular dysfunction in aged mice

Authors - Boying Zhao
mouseIF

EMBO J

Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex

Authors - Sharmin Naher
mouseIF

Dev Cell

Endoderm development requires centrioles to restrain p53-mediated apoptosis in the absence of ERK activity.

Authors - Chang Xie
mouseWB

Aging Cell

Excessive processing and acetylation of OPA1 aggravate age-related hearing loss via the dysregulation of mitochondrial dynamics

Authors - Andi Zhang
mouseWB

Biomed Pharmacother

Seno-antigen-pulsed dendritic cell vaccine induce anti-aging immunity to improve adipose tissue senescence and metabolic abnormalities

Authors - Yin Cao
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