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HRP-conjugated Chicken IgY Monoclonal antibody

Chicken IgY Monoclonal Antibody for WB

Host / Isotype

Mouse / IgG2a

Reactivity

Chicken

Applications

WB

Conjugate

HRP

CloneNo.

1E12G2

Cat no : HRP-67405

Print datasheet

Synonyms

验证数据展示

  • WB analysis of Recombinant protein using HRP-67405

    WB analysis of Recombinant protein using HRP-67405

    Lysate of Chicken egg were subjected to SDS PAGE followed by western blot with HRP-67405 (Chicken IgY antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inChicken egg

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

HRP-67405 targets Chicken IgY in WB applications and shows reactivity with Chicken samples.

Tested Applications WB Application Description
Tested Reactivity Chicken
Immunogen Peptide 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name Chicken IgY
Synonyms
Observed Molecular Weight 180 kDa, 70 kDa
Gene Symbol
Gene ID (NCBI)
RRIDAB_2883846
Conjugate HRP
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

IgY (immunoglobulin Y) is a type of immunoglobulin found in birds and reptiles. High concentration of IgY exists in chicken egg yolk. In chickens, IgY is functional same to IgG. IgY antibodies do not cross-react with Fc receptors, mammalian immunoglobulins such as rheumatoid factors and HAMA or activate the complement system thus could reduce false positive results in immunological assays. This antibody was generated from chicken IgY that extracted from chicken eggs. This product recognizes the heavry chain of checken IgY and can detect the contact form of chicken IgY (~180 kDa) and reduced form (~70 kDa).

实验方案

Product Specific Protocols
WB protocol for HRP Chicken IgY antibody HRP-67405Download protocol
Standard Protocols
Click here to view our Standard Protocols