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  • Featured Product
  • KD/KO Validated

Chromogranin B Polyclonal antibody, PBS Only

Chromogranin B Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

Cat no : 14968-1-PBS

Print datasheet

Synonyms

验证数据展示

  • WB analysis of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    WB analysis of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    PC-12 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

    WB analysis using 14968-1-AP (same clone as 14968-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHGB antibody included
  • IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    IHC staining of human pancreas using 14968-1-AP (same clone as 14968-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with CHGB antibody included
  • FC experiment of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    FC experiment of PC-12 using 14968-1-AP (same clone as 14968-1-PBS)

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (14968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    IF Staining of mouse colon using 14968-1-AP (same clone as 14968-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14968-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.

产品信息

14968-1-PBS targets Chromogranin B in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Chromogranin B fusion protein Ag6833 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromogranin B (secretogranin 1)
Synonyms
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 70-100 kDa
GenBank Accession NumberBC000375
Gene Symbol Chromogranin B
Gene ID (NCBI) 1114
RRIDAB_2081138
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05060
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa. It has been shown that CHGB has a clear degradation band at ~50 kDa (https://www.biorxiv.org/content/10.1101/2019.12.28.890053v1.full.pdf).