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Chromogranin B Polyclonal antibody

Chromogranin B Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 28663-1-AP

Print datasheet

Synonyms

CHGB, CgB, SCG1, Secretogranin 1, PE-11

验证数据展示

  • WB analysis using 28663-1-AP

    WB analysis using 28663-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 28663-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • FC experiment of PC-12 using 28663-1-AP

    FC experiment of PC-12 using 28663-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (28663-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inPC-12 cells
Positive FC (Intra) detected inPC-12 cells
Planning an IHC experiment? We recommend our IHCeasy CHGB Ready-To-Use IHC Kit. CHGB primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28663-1-AP targets Chromogranin B in WB, FC (Intra), ELISA applications and shows reactivity with human, rat samples.

Tested Applications WB, FC (Intra), ELISA Application Description
Tested Reactivity human, rat
Immunogen Chromogranin B fusion protein Ag29920 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name chromogranin B (secretogranin 1)
Synonyms CHGB, CgB, SCG1, Secretogranin 1, PE-11
Calculated Molecular Weight 78 kDa
Observed Molecular Weight 90-100 kDa
GenBank Accession NumberBC000375
Gene Symbol Chromogranin B
Gene ID (NCBI) 1114
RRIDAB_2881188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP05060
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for Chromogranin B antibody 28663-1-APDownload protocol
FC protocol for Chromogranin B antibody 28663-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols