• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis of COLO 320 using 25296-1-AP

    WB analysis of COLO 320 using 25296-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 25296-1-AP (Claudin 23 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 25296-1-AP

    IHC staining of human colon cancer using 25296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25296-1-AP (Claudin 23 antibody at dilution of 1:50 (under 10x lens).

  • IHC staining of human colon cancer using 25296-1-AP

    IHC staining of human colon cancer using 25296-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25296-1-AP (Claudin 23 antibody at dilution of 1:50 (under 40x lens).

Claudin 23 Polyclonal antibody

Claudin 23 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 25296-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human and More (1)

应用

WB, IHC, ELISA and More (1)

别名

claudin 23, CLDN23, CLDNL, hCG1646163

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive WB detected inCOLO 320 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25296-1-AP targets Claudin 23 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, canine
免疫原 Claudin 23 fusion protein Ag18189 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 claudin 23
别名 claudin 23, CLDN23, CLDNL, hCG1646163
计算分子量 292 aa, 32 kDa
观测分子量 32 kDa
GenBank蛋白编号BC125148
基因名称 Claudin 23
Gene ID (NCBI) 137075
RRIDAB_2880013
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96B33
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudin 23 (CLDN23) belongs to the large claudin family of proteins, which are tetraspan transmembrane proteins of tight junctions (PMID: 12736707; 18036336). Claudins exhibit specific patterns of expression in different tissues. Human CLDN23 mRNA was expressed in germinal center B cells, placenta, stomach as well as in colon tumor (PMID: 12736707). CLDN23 has been reported as a candidate tumor suppressor gene implicated in intestinal-type gastric cancer (PMID: 12736707).

实验方案

Product Specific Protocols
WB protocol for Claudin 23 antibody 25296-1-APDownload protocol
IHC protocol for Claudin 23 antibody 25296-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
human,canineWB,IF

Life Sci

Microbial metabolite n-butyrate upregulates intestinal claudin-23 expression through SP1 and AMPK pathways in mouse colon and human intestinal Caco-2 cells

Authors - Wenxi Xu
View Article
humanWB

Cell

snoRNA-facilitated protein secretion revealed by transcriptome-wide snoRNA target identification

Authors - Bei Liu
View Article