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Claudin 9 Polyclonal antibody

Claudin 9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30520-1-AP

Print datasheet

Synonyms

CLDN9

验证数据展示

  • WB analysis using 30520-1-AP

    WB analysis using 30520-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 30520-1-AP (Claudin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 30520-1-AP

    IHC staining of human endometrial cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 30520-1-AP

    IHC staining of human endometrial cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of T-47D using 30520-1-AP

    IF Staining of T-47D using 30520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Claudin 9 antibody (30520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHuH-7 cells
Positive IHC detected inhuman endometrial cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30520-1-AP targets Claudin 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen Claudin 9 fusion protein Ag29607 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name claudin 9
Synonyms CLDN9
Calculated Molecular Weight 217 aa, 23 kDa
Observed Molecular Weight21 kDa
GenBank Accession NumberBC051870
Gene Symbol Claudin 9
Gene ID (NCBI) 9080
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95484
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.

实验方案

Product Specific Protocols
WB protocol for Claudin 9 antibody 30520-1-APDownload protocol
IHC protocol for Claudin 9 antibody 30520-1-APDownload protocol
IF protocol for Claudin 9 antibody 30520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols