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验证数据展示

  • WB analysis using 30520-1-AP

    WB analysis using 30520-1-AP

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 30520-1-AP (Claudin 9 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human endometrial cancer using 30520-1-AP

    IHC staining of human endometrial cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human endometrial cancer using 30520-1-AP

    IHC staining of human endometrial cancer using 30520-1-AP

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 30520-1-AP (Claudin 9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of T-47D using 30520-1-AP

    IF Staining of T-47D using 30520-1-AP

    Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Claudin 9 antibody (30520-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

Claudin 9 Polyclonal antibody

Claudin 9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 30520-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CLDN9

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHuH-7 cells
Positive IHC detected inhuman endometrial cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inT-47D cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30520-1-AP targets Claudin 9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 Claudin 9 fusion protein Ag29607 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 claudin 9
别名 CLDN9
计算分子量 217 aa, 23 kDa
观测分子量21 kDa
GenBank蛋白编号BC051870
基因名称 Claudin 9
Gene ID (NCBI) 9080
RRIDAB_3086347
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95484
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium.They are small (20-27kDa) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 9.

实验方案

Product Specific Protocols
WB protocol for Claudin 9 antibody 30520-1-APDownload protocol
IHC protocol for Claudin 9 antibody 30520-1-APDownload protocol
IF protocol for Claudin 9 antibody 30520-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols